FN Archimer Export Format
PT J
TI Sex matters in massive parallel sequencing: Evidence for biases in genetic parameter estimation and investigation of sex determination systems
BT 
AF BENESTAN, Laura
   MOORE, Jean-Sebastien
   SUTHERLAND, Ben J. G.
   LE LUYER, Jeremy
   MAAROUFI, Halim
   ROUGEUX, Clement
   NORMANDEAU, Eric
   RYCROFT, Nathan
   ATEMA, Jelle
   HARRIS, Les N.
   TALLMAN, Ross F.
   GREENWOOD, Spencer J.
   CLARK, Fraser K.
   BERNATCHEZ, Louis
AS 1:1,2;2:1,2;3:1,2;4:1,2;5:2;6:1,2;7:1,2;8:3;9:3;10:4;11:4;12:5,6;13:5,6;14:1,2;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:;13:;14:;
C1 Univ Laval, Dept Biol, Quebec City, PQ, Canada.
   Univ Laval, IBIS, Quebec City, PQ, Canada.
   Boston Univ, Dept Biol, 5 Cummington St, Boston, MA 02215 USA.
   Fisheries & Oceans Canada, Freshwater Inst, Winnipeg, MB, Canada.
   Univ Prince Edward Isl, Dept Biomed Sci, Atlantic Vet Coll, Charlottetown, PE, Canada.
   Univ Prince Edward Isl, AVC Lobster Sci Ctr, Atlantic Vet Coll, Charlottetown, PE, Canada.
C2 UNIV LAVAL, CANADA
   UNIV LAVAL, CANADA
   UNIV BOSTON, USA
   MPO, CANADA
   UNIV PRINCE EDWARD ISL, CANADA
   UNIV PRINCE EDWARD ISL, CANADA
IF 6.131
TC 35
UR https://archimer.ifremer.fr/doc/00409/52046/108142.pdf
LA English
DT Article
DE ;conservation genetics;fisheries management;genotyping-by-sequencing;marine genomics;RADseq
AB Using massively parallel sequencing data from two species with different life history traits, American lobster (Homarus americanus) and Arctic Char (Salvelinus alpinus), we highlight how an unbalanced sex ratio in the samples and a few sex-linked markers may lead to false interpretations of population structure and thus to potentially erroneous management recommendations. Here, multivariate analyses revealed two genetic clusters separating samples by sex instead of by expected spatial variation: inshore and offshore locations in lobster, or east and west locations in Arctic Char. To further investigate this, we created several subsamples artificially varying the sex ratio in the inshore/offshore and east/west groups and then demonstrated that significant genetic differentiation could be observed despite panmixia in lobster, and that FST values were overestimated in Arctic Char. This pattern was due to 12 and 94 sex-linked markers driving differentiation for lobster and Arctic Char, respectively. Removing sex-linked markers led to nonsignificant genetic structure in lobster and a more accurate estimation of FST in Arctic Char. The locations of these markers and putative identities of genes containing or nearby the markers were determined using available transcriptomic and genomic data, and this provided new information related to sex determination in both species. Given that only 9.6% of all marine/diadromous population genomic studies to date have reported sex information, we urge researchers to collect and consider individual sex information. Sex information is therefore relevant for avoiding unexpected biases due to sex-linked markers as well as for improving our knowledge of sex determination systems in nonmodel species. 
PY 2017
PD DEC
SO Molecular Ecology
SN 0962-1083
PU Wiley
VL 26
IS 24
UT 000419045000002
BP 6767
EP 6783
DI 10.1111/mec.14217
ID 52046
ER

EF