FN Archimer Export Format PT J TI The combined effects of blue light and dilution rate on lipid class and fatty acid composition of Tisochrysis lutea BT AF MARCHETTI, Julie DA COSTA, Fiz BOUGARAN, Gael QUERE, Claudie SOUDANT, Philippe ROBERT, Rene AS 1:1;2:2;3:1;4:3;5:4;6:5; FF 1:;2:;3:PDG-RBE-BRM-LPBA;4:PDG-RBE-PFOM-LPI;5:;6:PDG-ODE-LITTORAL; C1 IFREMER, Lab Physiol & Biotechnol Algues, Rue Lile Yeu,BP 21105, F-44311 Nantes 3, France. Oceano Fresco Lda, Edificio Mira Ctr, Rua Matadouro,Piso 2,Lab B2, P-3070436 Mira, Portugal. IFREMER, Lab Sci Environm Marin, UMR 6539, LEMAR, F-29280 Plouzane, France. UBO, IUEM, UMR 6539, Lab Sci Environm Marin,LEMAR, Technopole Brest Iroise, Plouzane, France. IFREMER, Unite Littoral, Ctr Bretagne, Pointe Diable CS 10070, F-29280 Plouzane, ZI, France. C2 IFREMER, FRANCE OCEANO FRESCO LDA, PORTUGAL IFREMER, FRANCE UBO, FRANCE IFREMER, FRANCE SI NANTES BREST SE PDG-RBE-BRM-LPBA PDG-RBE-PFOM-LPI PDG-ODE-LITTORAL UM LEMAR IN WOS Ifremer jusqu'en 2018 copubli-france copubli-europe copubli-univ-france IF 2.635 TC 16 UR https://archimer.ifremer.fr/doc/00412/52299/53355.pdf LA English DT Article DE ;Tisochrysis lutea;Blue light;Dilution rate;Lipid classes;Fatty acids;Chemostat AB The lipid class and the fatty acid compositions of microalgae highly influence bivalve larval and post-larval development. Light is an essential environmental factor for microalgal culture, and quantity and quality of light may induce changes in the biochemical composition of the algae. The objective of this study was to investigate the effect of light spectrum (blue vs. white light) on lipid class and fatty acid compositions of Tisochrysis lutea cultured in a chemostat. Two different dilution rates (D) were assayed for each light spectrum: 0.2 and 0.7 day−1. Triacylglycerol (TAG), sterol, and hydrocarbon (HC) content increased sharply at low D. The proportion of alkenones was significantly reduced under blue light. Polyunsaturated fatty acids (PUFA), and particularly n-3 PUFA, content in phospholipids (PL) increased under blue light compared to white light at low D. Thus, blue light raised 22:6(n-3) levels in total lipids of T. lutea at low D. The cultivation of T. lutea in a chemostat at low D under blue light may improve nutritional value as feed for bivalve larvae by modifying the PUFA profile, especially increasing 22:6(n-3). PY 2018 PD JUL SO Journal Of Applied Phycology SN 0921-8971 PU Springer VL 30 IS 3 UT 000433966200004 BP 1483 EP 1494 DI 10.1007/s10811-017-1340-y ID 52299 ER EF