FN Archimer Export Format
PT J
TI Organometallic nanoprobe to enhance optical response on the polycyclic aromatic hydrocarbon benzo[a]pyrene immunoassay using SERS technology
BT 
AF DRIBEK, Mohamed
   RINNERT, Emmanuel
   COLAS, Florent
   CRASSOUS, Marie-Pierre
   THIOUNE, Nene
   DAVID, Catalina
   DE LA CHAPELLE, Marc
   COMPERE, Chantal
AS 1:1,3;2:1;3:1;4:1;5:2;6:2,4;7:2;8:1;
FF 1:;2:PDG-REM-GM-LCG;3:PDG-REM-RDT-LDCM;4:PDG-REM-RDT-LDCM;5:;6:;7:;8:PDG-REM-RDT;
C1 IFREMER, Lab Detect Capteurs & Mesures, CS10070, F-29280 Plouzane, France.
   Univ Paris 13, UFR SMBH, Lab CSPBAT FRE 3043, 74 Rue Marcel Cachin, F-93017 Bobigny, France.
   Univ Francois Rabelais Tours, UFR Sci Pharmaceut, EA Nanomedicaments & Nanosondes 6295, 31 Ave Monge, F-37200 Tours, France.
   Horiba Sci, 231 Rue Lille, F-59650 Villeneuve Dascq, France.
C2 IFREMER, FRANCE
   UNIV PARIS 13, FRANCE
   UNIV TOURS, FRANCE
   HORIBA SCI, FRANCE
SI BREST
SE PDG-REM-GM-LCG
   PDG-REM-RDT-LDCM
   PDG-REM-RDT
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 2.8
TC 17
UR https://archimer.ifremer.fr/doc/00417/52844/53870.pdf
LA English
DT Article
DE ;Biosensor;Surface-enhanced Raman scattering;Polycyclic aromatic hydrocarbons;Benzo[a]pyrene antibody;Organometallic nanoprobe
AB We demonstrated the use of a new organometallic nanoprobe for competitive surface-enhanced Raman scattering (SERS) immunoassay devoted to the detection of polycyclic aromatic hydrocarbons (PAH) such as benzo[a]pyrene (BaP) in seawater. The nanoprobes are gold nanoparticles (GNPs) labeled by a Raman reporter, the 5,5'-dithiobis(succinimidyl-2-nitrobenzoate) (DSNB) and functionalized with monoclonal antibodies anti-BaP. The antibodies are bound with a high specificity to the analyte while the GNPs enhanced the Raman scattering of the DSNB. This type of immunoassay involved the grafting of BaP onto a sensing surface. Thus, NH2-terminated self-assembled monolayer is formed on the surface of gold substrate using cysteamine. Amines finally reacted with 6-formylbenzo[a]pyrene. So, this SERS detection involves four steps: (i) the nanoprobes are incubated with the sample; (ii) a drop of the mixture is then put onto the substrate; (iii) the surface is rinsed; and (iv) the surface is analyzed by Raman spectroscopy. To synthesize the nanoprobes, firstly, we prepared GNPs according to Frens' method. Then, GNPs were spontaneously labeled by the DSNB Raman reporter, thanks to a strong gold-sulfur interaction. Thereafter, BaP antibodies were cross-linked to the DSNB labeled GNPs by reaction of proteins primary amino groups with N-hydroxyl succinimide (NHS). Before use in SERS detection, their activity was controlled by surface plasmon resonance technique. The present method allows us to detect BaP at trace concentration (2 nmol/L). The results demonstrate that the proposed method has a great potential for application in the monitoring of seawater.
PY 2017
PD DEC
SO Environmental Science And Pollution Research
SN 0944-1344
PU Springer Heidelberg
VL 24
IS 35
UT 000417545800015
BP 27070
EP 27076
DI 10.1007/s11356-014-3384-8
ID 52844
ER

EF