FN Archimer Export Format PT J TI The Voltage-Dependent Anion Channel (VDAC) of Pacific Oysters Crassostrea gigas Is Upaccumulated During Infection by the Ostreid Herpesvirus-1 (OsHV-1): an Indicator of the Warburg Effect BT AF DELISLE, Lizenn FUHRMANN, Marine QUERE, Claudie PAULETTO, Marianna PICHEREAU, Vianney PERNET, Fabrice CORPOREAU, Charlotte AS 1:1,2;2:1;3:1;4:3;5:4;6:1;7:1; FF 1:PDG-RBE-PFOM-LPI;2:PDG-ODE-DYNECO-LEBCO;3:PDG-RBE-PFOM-LPI;4:;5:;6:PDG-RBE-PFOM-LPI;7:PDG-RBE-PFOM-LPI; C1 IFREMER, CNRS, Lab Sci Environm Marin LEMAR, UBO,IRD,UMR 6539, F-29280 Plouzane, France. IFREMER, Ctr Ifremer Bretagne, Unite Physiol Fonct Organ Marins PFOM, LPI, 1625 Route St Anne,CS 10070, F-29280 Plouzane, France. Univ Padua, Dept Comparat Biomed & Food Sci, Viale Univ 16, I-35020 Padua, Italy. Univ Bretagne Occidentale, CNRS, IFREMER, Lab Sci Environm Marin LEMAR,UBO,IRD,UMR 6539, F-29280 Plouzane, France. C2 IFREMER, FRANCE IFREMER, FRANCE UNIV PADUA, ITALY UBO, FRANCE SI BREST SE PDG-RBE-PFOM-LPI PDG-ODE-DYNECO-LEBCO UM LEMAR IN WOS Ifremer jusqu'en 2018 copubli-france copubli-europe copubli-univ-france IF 2.798 TC 8 UR https://archimer.ifremer.fr/doc/00420/53129/54142.pdf LA English DT Article DE ;Voltage-dependent anion channel;Warburg effect;Crassostrea gigas;Ostreid herpes virus AB Voltage-dependent anion channel (VDAC) is a key mitochondrial protein. VDAC drives cellular energy metabolism by controlling the influx and efflux of metabolites and ions through the mitochondrial membrane, playing a role in its permeabilization. This protein exerts a pivotal role during the white spot syndrome virus (WSSV) infection in shrimp, through its involvement in a particular metabolism that plays in favor of the virus, the Warburg effect. The Warburg effect corresponds to an atypical metabolic shift toward an aerobic glycolysis that provides energy for rapid cell division and resistance to apoptosis. In the Pacific oyster Crassostrea gigas, the Warburg effect occurs during infection by Ostreid herpesvirus (OsHV-1). At present, the role of VDAC in the Warburg effect, OsHV-1 infection and apoptosis is unknown. Here, we developed a specific antibody directed against C. gigas VDAC. This tool allowed us to quantify the tissue-specific expression of VDAC, to detect VDAC oligomers, and to follow the amount of VDAC in oysters deployed in the field. We showed that oysters sensitive to a mortality event in the field presented an accumulation of VDAC. Finally, we propose to use VDAC quantification as a tool to measure the oyster susceptibility to OsHV-1 depending on its environment. PY 2018 PD FEB SO Marine Biotechnology SN 1436-2228 PU Springer VL 20 IS 1 UT 000424459500008 BP 87 EP 97 DI 10.1007/s10126-017-9789-x ID 53129 ER EF