FN Archimer Export Format
PT J
TI Cryopreservation of Pacific oyster ( Crassostrea gigas ) larvae: Revisiting the practical limitations and scaling up the procedure for application to hatchery
BT 
AF LABBE, Catherine
   HAFFRAY, Pierrick
   MINGANT, Christian
   QUITTET, Benjamin
   DISS, Blandine
   TERVIT, H. Robin
   ADAMS, Serean L.
   RIMOND, Flore
   SUQUET, Marc
AS 1:1;2:2;3:3;4:2;5:4;6:5;7:6;8:3;9:3;
FF 1:;2:;3:PDG-RBE-PFOM-LPI;4:;5:;6:;7:;8:;9:PDG-RBE-PFOM-LPI;
C1 INRA, LPGP, UR1037, Rennes, France.
   LPGP, SYSAAF, Rennes, France.
   IFREMER, PFOM Dept, Stn Expt Argenton, UMR 6539, Argenton, France.
   Satmar, Barfleur, France.
   AgResearch, Private Bag 3123, Hamilton, New Zealand.
   Cawthron Inst, Hamilton, New Zealand.
C2 INRA, FRANCE
   SYSAAF, FRANCE
   IFREMER, FRANCE
   SATMAR, FRANCE
   AGRESEARCH, NEW ZEALAND
   CAWTHRON INST, NEW ZEALAND
SI BREST
   ARGENTON
SE PDG-RBE-PFOM-LPI
UM LEMAR
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-p187
   copubli-int-hors-europe
IF 3.022
TC 17
UR https://archimer.ifremer.fr/doc/00423/53460/54354.pdf
LA English
DT Article
DE ;Oyster;Cryobanking;D stage;Ethylene glycol;High throughput;Hatchery
AB Pacific oyster Crassostrea gigas is one major species for aquaculture, and the development of breeding programs and the need for preservation of wild stock genetic resources prompted the need for larvae cryopreservation. The objective of the present study was to choose the most reliable protocol from several existing publications, to test its biological and practical limitations, and to adapt it to hatchery conditions. The selected protocol was characterized by a very slow freezing rate without seeding, and by the use of ethylene glycol and sucrose as cryoprotectant. The best survivals after thawing and rearing up to 48 h post fertilization (hpf) were obtained with larvae that were frozen at late trochophore (20 hpf) and early-D (24 hpf) stages. Increasing the larvae concentration in the straws and using high throughput straw filling and freezing devices did not alter the cryopreservation outcome. The whole procedure was applied to cryopreservation in a commercial hatchery (Satmar, France), and the thawed larvae yielded 9.4 ± 4.5% survivals at 12 days post fertilization. The overall success was dampened by some variability in the larvae survival that is likely due to the physiological status of the larvae. In all, the proposed procedure is robust and reliable and can be used for cryobanking of oyster genetic resources. 
PY 2018
PD MAR
SO Aquaculture
SN 0044-8486
PU Elsevier Science Bv
VL 488
UT 000428685800026
BP 227
EP 234
DI 10.1016/j.aquaculture.2018.01.023
ID 53460
ER

EF