Bile Salt Hydrolase Activities: A Novel Target to Screen Anti-Giardia Lactobacilli?

Type Article
Date 2018-02
Language English
Author(s) Allain Thibault1, 2, Chaouch Soraya2, Thomas Myriam3, 4, Travers Marie-AgnesORCID, Valle Isabelle3, 4, Langella Philippe1, Grellier Philippe2, Polack Bruno3, 4, Florent Isabelle2, Bermudez-Humaran Luis G.1
Affiliation(s) 1 : AgroParisTech, INRA, Micalis Inst, Commensal & Probiot Host Interact Lab, Paris, France.
2 : Sorbonne Univ, Ctr Natl Rech Sci, Museum Natl Hist Nat, UMR 7245, Paris, France.
3 : Univ Paris Est, INRA, ENVA, BIPAR,ANSES, Champs Sur Marne, France.
4 : ANSES, INRA, ENVA, BIPAR,Lab Sante Anim, Maisons Alfort, France.
Source Frontiers In Microbiology (1664-302X) (Frontiers Media Sa), 2018-02 , Vol. 9 , N. 89 , P. 9p.
DOI 10.3389/fmicb.2018.00089
WOS© Times Cited 20
Keyword(s) Giardia duodenalis, lactobacilli, Lactobacillus johnsonii, Lactobacillus gasseri, probiotics, bile salt hydrolases

Giardia duodenalis is a protozoan parasite responsible for giardiasis, a disease characterized by intestinal malabsorption, diarrhea and abdominal pain in a large number of mammal species. Giardiasis is one of the most common intestinal parasitic diseases in the world and thus a high veterinary, and public health concern. It is wellestablished that some probiotic bacteria may confer protection against this parasite in vitro and in vivo and we recently documented the implication of bile-salt hydrolase (BSH)-like activities from strain La1 of Lactobacillus johnsonii as mediators of these effects in vitro. We showed that these activities were able to generate deconjugated bile salts that were toxic to the parasite. In the present study, a wide collection of lactobacilli strains from different ecological origins was screened to assay their antigiardial effects. Our results revealed that the anti-parasitic effects of some of the strains tested were well-correlated with the expression of BSH-like activities. The two most active strains in vitro, La1 and Lactobacillus gasseri  CNCM I-4884, were then tested for their capacity to influence G. duodenalis infection in a suckling mice model. Strikingly, only L. gasseri CNCM I-4884 strain was able to significantly antagonize parasite growth with a dramatic reduction of the trophozoites load in the small intestine. Moreover, this strain also significantly reduced the fecal excretion of Giardia cysts after 5 days of treatment, which could contribute to blocking the transmission of the parasite, in contrast of La1 where no effect was observed. This study represents a step toward the development of new prophylactic strategies to combat G. duodenalis infection in both humans and animals

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FIGURE S1 Persistence of L. johnsonii La1 strain (Eryr) in OF1 suckling mice. Each mouse received a single administration of 5 × 108 CFU of L. johnsonii La1 Eryr either by oral gavage... 325 KB Open access
FIGURE S2 Kinetics of infection of G. duodenalis strain WB6 in OF1 suckling mice. (A) G. duodenalis trophozoites enumeration in small intestine after single gavage (day 0). Trophozoite burden ... 711 KB Open access
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Allain Thibault, Chaouch Soraya, Thomas Myriam, Travers Marie-Agnes, Valle Isabelle, Langella Philippe, Grellier Philippe, Polack Bruno, Florent Isabelle, Bermudez-Humaran Luis G. (2018). Bile Salt Hydrolase Activities: A Novel Target to Screen Anti-Giardia Lactobacilli? Frontiers In Microbiology, 9(89), 9p. Publisher's official version : , Open Access version :