TY - JOUR T1 - Bile Salt Hydrolase Activities: A Novel Target to Screen Anti-Giardia Lactobacilli? A1 - Allain,Thibault A1 - Chaouch,Soraya A1 - Thomas,Myriam A1 - Travers,Marie-Agnes A1 - Valle,Isabelle A1 - Langella,Philippe A1 - Grellier,Philippe A1 - Polack,Bruno A1 - Florent,Isabelle A1 - Bermudez-Humaran,Luis G. AD - AgroParisTech, INRA, Micalis Inst, Commensal & Probiot Host Interact Lab, Paris, France. AD - Sorbonne Univ, Ctr Natl Rech Sci, Museum Natl Hist Nat, UMR 7245, Paris, France. AD - Univ Paris Est, INRA, ENVA, BIPAR,ANSES, Champs Sur Marne, France. AD - ANSES, INRA, ENVA, BIPAR,Lab Sante Anim, Maisons Alfort, France. UR - https://archimer.ifremer.fr/doc/00424/53569/ DO - 10.3389/fmicb.2018.00089 KW - Giardia duodenalis KW - lactobacilli KW - Lactobacillus johnsonii KW - Lactobacillus gasseri KW - probiotics KW - bile salt hydrolases N2 - Giardia duodenalis is a protozoan parasite responsible for giardiasis, a disease characterized by intestinal malabsorption, diarrhea and abdominal pain in a large number of mammal species. Giardiasis is one of the most common intestinal parasitic diseases in the world and thus a high veterinary, and public health concern. It is wellestablished that some probiotic bacteria may confer protection against this parasite in vitro and in vivo and we recently documented the implication of bile-salt hydrolase (BSH)-like activities from strain La1 of Lactobacillus johnsonii as mediators of these effects in vitro. We showed that these activities were able to generate deconjugated bile salts that were toxic to the parasite. In the present study, a wide collection of lactobacilli strains from different ecological origins was screened to assay their antigiardial effects. Our results revealed that the anti-parasitic effects of some of the strains tested were well-correlated with the expression of BSH-like activities. The two most active strains in vitro, La1 and Lactobacillus gasseriĀ  CNCM I-4884, were then tested for their capacity to influence G. duodenalis infection in a suckling mice model. Strikingly, only L. gasseri CNCM I-4884 strain was able to significantly antagonize parasite growth with a dramatic reduction of the trophozoites load in the small intestine. Moreover, this strain also significantly reduced the fecal excretion of Giardia cysts after 5 days of treatment, which could contribute to blocking the transmission of the parasite, in contrast of La1 where no effect was observed. This study represents a step toward the development of new prophylactic strategies to combat G. duodenalis infection in both humans and animals Y1 - 2018/02 PB - Frontiers Media Sa JF - Frontiers In Microbiology SN - 1664-302X VL - 9 IS - 89 ID - 53569 ER -