pH controls spermatozoa motility in the Pacific oyster ( Crassostrea gigas )

Type Article
Date 2018-03
Language English
Author(s) Boulais Myrina1, 2, Suquet Marc2, Arsenault-Pernet Marie Eve JulieORCID2, Malo Florent2, Queau Isabelle2, Pignet Patricia3, Ratiskol Dominique2, Le Grand Jacqueline2, Huber Matthias2, Cosson Jacky4
Affiliation(s) 1 : IUEM, IFREMER, IRD, CNRS,UBO,UMR Lemar 6539, F-29280 Plouzane, France.
2 : IFREMER, IRD, CNRS, UBO,UMR Lemar 6539, Site Expt Argenton, F-29840 Landunvez, France.
3 : IFREMER, Ctr Bretagne, LMEE, F-29280 Plouzane, France.
4 : Univ South Bohemia Ceske Budejovice, South Bohemian Res Ctr Aquaculture & Biodivers Hy, Fac Fisheries & Protect Waters, Vodnany 38925, Czech Republic.
Source Biology Open (2046-6390) (Company Of Biologists Ltd), 2018-03 , Vol. 7 , N. 3 , P. bio031427 (8p.)
DOI 10.1242/bio.031427
WOS© Times Cited 9
Keyword(s) Seminal fluid, Spermatozoa, Motility, pH, Salinity, Ions
Abstract

Investigating the roles of chemical factors stimulating and inhibiting sperm motility is required to understand the mechanisms of spermatozoa movement. In this study, we described the composition of the seminal fluid (osmotic pressure, pH, and ions) and investigated the roles of these factors and salinity in initiating spermatozoa movement in the Pacific oyster. The acidic pH of the gonad (5.82 ± 0.22) maintained sperm in the quiescent stage and initiation of flagellar movement was triggered by a sudden increase of spermatozoa external pH (pHe) when released in seawater (SW). At pH 6.4, percentage of motile spermatozoa was three times higher when they were activated in SW containing 30 mM NH4Cl, which alkalinizes internal pH (pHi) of spermatozoa, compared to NH4Cl-free SW, revealing the role of pHi in triggering sperm movement. Percentage of motile spermatozoa activated in Na+-free artificial seawater (ASW) was highly reduced compared to ASW, suggesting that change of pHi triggering sperm motility was mediated by a Na+/H+ exchanger. Motility and swimming speed were highest in salinities between 33.8 and 42.7‰ (within a range of 0 to 50 ‰), and pH values above 7.5 (within a range of 4.5 to 9.5).

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Boulais Myrina, Suquet Marc, Arsenault-Pernet Marie Eve Julie, Malo Florent, Queau Isabelle, Pignet Patricia, Ratiskol Dominique, Le Grand Jacqueline, Huber Matthias, Cosson Jacky (2018). pH controls spermatozoa motility in the Pacific oyster ( Crassostrea gigas ). Biology Open, 7(3), bio031427 (8p.). Publisher's official version : https://doi.org/10.1242/bio.031427 , Open Access version : https://archimer.ifremer.fr/doc/00428/53976/