FN Archimer Export Format
PT J
TI pH controls spermatozoa motility in the Pacific oyster (     Crassostrea gigas     )
BT 
AF BOULAIS, Myrina
   SUQUET, Marc
   ARSENAULT-PERNET, Marie Eve Julie
   MALO, Florent
   QUEAU, Isabelle
   PIGNET, Patricia
   RATISKOL, Dominique
   LE GRAND, Jacqueline
   HUBER, Matthias
   COSSON, Jacky
AS 1:1,2;2:2;3:2;4:2;5:2;6:3;7:2;8:2;9:2;10:4;
FF 1:;2:PDG-RBE-PFOM-LPI;3:PDG-REM-EEP-LEP;4:;5:PDG-RBE-PFOM-LPI;6:PDG-REM-EEP-LMEE;7:PDG-RBE-PFOM-LPI;8:PDG-RBE-PFOM-LPI;9:PDG-RBE-PFOM-LPI;10:;
C1 IUEM, IFREMER, IRD, CNRS,UBO,UMR Lemar 6539, F-29280 Plouzane, France.
   IFREMER, IRD, CNRS, UBO,UMR Lemar 6539, Site Expt Argenton, F-29840 Landunvez, France.
   IFREMER, Ctr Bretagne, LMEE, F-29280 Plouzane, France.
   Univ South Bohemia Ceske Budejovice, South Bohemian Res Ctr Aquaculture & Biodivers Hy, Fac Fisheries & Protect Waters, Vodnany 38925, Czech Republic.
C2 UBO, FRANCE
   IFREMER, FRANCE
   IFREMER, FRANCE
   UNIV S BOHEMIA, CZECH REPUBLIC
SI ARGENTON
   BREST
   NANTES
SE PDG-RBE-PFOM-LPI
   PDG-REM-EEP-LEP
   PDG-REM-EEP-LMEE
UM BEEP-LM2E
   LEMAR
IN WOS Ifremer jusqu'en 2018
   DOAJ
   copubli-france
   copubli-europe
   copubli-univ-france
IF 1.962
TC 21
UR https://archimer.ifremer.fr/doc/00428/53976/55137.pdf
LA English
DT Article
DE ;Seminal fluid;Spermatozoa;Motility;pH;Salinity;Ions
AB Investigating the roles of chemical factors stimulating and inhibiting sperm motility is required to understand the mechanisms of spermatozoa movement. In this study, we described the composition of the seminal fluid (osmotic pressure, pH, and ions) and investigated the roles of these factors and salinity in initiating spermatozoa movement in the Pacific oyster. The acidic pH of the gonad (5.82 ± 0.22) maintained sperm in the quiescent stage and initiation of flagellar movement was triggered by a sudden increase of spermatozoa external pH (pHe) when released in seawater (SW). At pH 6.4, percentage of motile spermatozoa was three times higher when they were activated in SW containing 30 mM NH4Cl, which alkalinizes internal pH (pHi) of spermatozoa, compared to NH4Cl-free SW, revealing the role of pHi in triggering sperm movement. Percentage of motile spermatozoa activated in Na+-free artificial seawater (ASW) was highly reduced compared to ASW, suggesting that change of pHi triggering sperm motility was mediated by a Na+/H+ exchanger. Motility and swimming speed were highest in salinities between 33.8 and 42.7‰ (within a range of 0 to 50 ‰), and pH values above 7.5 (within a range of 4.5 to 9.5). 
PY 2018
PD MAR
SO Biology Open
SN 2046-6390
PU Company Of Biologists Ltd
VL 7
IS 3
UT 000429100500005
DI 10.1242/bio.031427
ID 53976
ER

EF