The calibration of multibeam echosounders for backscatter measurements can be conducted efficiently and accurately using data from surveys over a reference natural area, implying appropriate measurements of the local absolute values of backscatter. Such a shallow area (20-m mean depth) has been defined and qualified in the Bay of Brest (France), and chosen as a reference area for multibeam systems operating at 200 and 300 kHz. The absolute reflectivity over the area was measured using a calibrated single-beam fishery echosounder (Simrad EK60) tilted at incidence angles varying between 0° and 60° with a step of 3°. This reference backscatter level is then compared to the average backscatter values obtained by a multibeam echosounder (here a Kongsberg EM 2040-D) at a close frequency and measured as a function of angle; the difference gives the angular bias applicable to the multibeam system for recorded level calibration. The method is validated by checking the single- and multibeam data obtained on other areas with sediment types different from the reference area.