FN Archimer Export Format
PT J
TI Toxicological Investigations on the Sea Urchin Tripneustes gratilla (Toxopneustidae, Echinoid) from Anaho Bay (Nuku Hiva, French Polynesia): Evidence for the Presence of Pacific Ciguatoxins
BT 
AF DARIUS, Helene Taiana
   ROUE, Melanie
   SIBAT, Manoella
   VIALLON, Jerome
   GATTI, Clemence Mahana Iti
   VANDERSEA, Mark W.
   TESTER, Patricia A.
   LITAKER, R. Wayne
   AMZIL, Zouher
   HESS, Philipp
   CHINAIN, Mireille
AS 1:1;2:2;3:3;4:1;5:1;6:4;7:5;8:4;9:3;10:3;11:1;
FF 1:;2:;3:PDG-ODE-DYNECO-PHYC;4:;5:;6:;7:;8:;9:PDG-ODE-DYNECO-PHYC;10:PDG-ODE-DYNECO-PHYC;11:;
C1 ILM, Lab Tox Microalgae, UMR 241, EIO, POB 30, F-98713 Tahiti, French Polynesi, France.
   IRD, UMR 241, EIO, POB 53267, F-98716 Tahiti, French Polynesi, France.
   IFREMER, Phycotoxins Lab, F-44311 Nantes, France.
   Natl Ocean Serv, Natl Ocean & Atmospher Adm, Ctr Coastal Ocean Sci, Beaufort Lab, Beaufort, NC 28516 USA.
   Ocean Tester LLC, Beaufort, NC 28516 USA.
C2 INST LOUIS MALARDE, FRANCE
   IRD, FRANCE
   IFREMER, FRANCE
   NOAA, USA
   OCEAN TESTER LLC, USA
SI NANTES
SE PDG-ODE-DYNECO-PHYC
UM EIO
IN WOS Ifremer jusqu'en 2018
   DOAJ
   copubli-france
   copubli-p187
   copubli-int-hors-europe
IF 3.772
TC 37
UR https://archimer.ifremer.fr/doc/00436/54716/56153.pdf
LA English
DT Article
DE ;ciguatera poisoning;ciguatoxins;Tripneustes gratilla;sea urchin;Echinoidea;Gambierdiscus polynesiensis;windowscreens;artificial substrates;qPCR assays;CBA-N2a;LC-MS/MS
AB The sea urchin Tripneustes gratilla (Toxopneustidae, Echinoids) is a source of protein for many islanders in the Indo-West Pacific. It was previously reported to occasionally cause ciguatera-like poisoning; however, the exact nature of the causative agent was not confirmed. In April and July 2015, ciguatera poisonings were reported following the consumption of T. gratilla in Anaho Bay (Nuku Hiva Island, Marquesas archipelago, French Polynesia). Patient symptomatology was recorded and sea urchin samples were collected from Anaho Bay in July 2015 and November 2016. Toxicity analysis using the neuroblastoma cell–based assay (CBA-N2a) detected the presence of ciguatoxins (CTXs) in T. gratilla samples. Gambierdiscus species were predominant in the benthic assemblages of Anaho Bay, and G. polynesiensis was highly prevalent in in vitro cultures according to qPCR results. Liquid chromatography–tandem mass spectrometry (LC-MS/MS) analyses revealed that P-CTX-3B was the major ciguatoxin congener in toxic sea urchin samples, followed by 51-OH-P-CTX-3C, P-CTX-3C, P-CTX-4A, and P-CTX-4B. Between July 2015 and November 2016, the toxin content in T. gratilla decreased, but was consistently above the safety limit allowed for human consumption. This study provides evidence of CTX bioaccumulation in T. gratilla as a cause of ciguatera-like poisoning associated with a documented symptomatology. 
PY 2018
PD APR
SO Marine Drugs
SN 1660-3397
PU Mdpi
VL 16
IS 4
UT 000431068000024
DI 10.3390/md16040122
ID 54716
ER

EF