FN Archimer Export Format
PT J
TI A new diatom species P. hallegraeffii sp. nov. belonging to the toxic genus Pseudo-nitzschia (Bacillariophyceae) from the East Australian Current
BT 
AF AJANI, Penelope A.
   VERMA, Arjun
   LASSUDRIE, Malwenn
   DOBLIN, Martina A.
   MURRAY, Shauna A.
AS 1:1;2:1;3:1;4:1;5:1;
FF 1:;2:;3:;4:;5:;
C1 Univ Technol Sydney, C3, Broadway, NSW, Australia.
C2 UNIV TECHNOL SYDNEY, AUSTRALIA
IN DOAJ
IF 2.776
TC 22
UR https://archimer.ifremer.fr/doc/00436/54794/56248.pdf
   https://archimer.ifremer.fr/doc/00436/54794/56249.tif
   https://archimer.ifremer.fr/doc/00436/54794/56250.pdf
   https://archimer.ifremer.fr/doc/00436/54794/56251.xls
   https://archimer.ifremer.fr/doc/00436/54794/56252.xls
LA English
DT Article
AB A new species belonging to the toxin producing diatom genus Pseudo-nitzschia, P. hallegraeffii sp. nov., is delineated and described from the East Australian Current (EAC). Clonal cultures were established by single cell isolation from phytoplankton net hauls collected as part of a research expedition in the EAC region in 2016 on the RV Investigator. Cultures were assessed for their morphological and genetic characteristics, their sexual compatibility with other Pseudo-nitzschia species, and their ability to produce domoic acid. Light and transmission electron microscopy revealed cells which differed from their closest relatives by their cell width, rows of poroids, girdle band structure and density of band straie. Phylogenetic analyses based on sequencing of nuclear-encoded ribosomal deoxyribonucleic acid (rDNA) regions showed this novel genotype clustered within the P. delicatissima complex, but formed a discrete clade from its closest relatives P. dolorosa, P. simulans, P. micropora and P. delicatissima. Complementary base changes (CBCs) were observed in the secondary structure of the 3’ nuclear ribosomal transcribed spacer sequence region (ITS2) between P. hallegraeffii sp. nov. and its closest related taxa, P. simulans and P. dolorosa. Under laboratory conditions, and in the absence of any zooplankton cues, strains of P. hallegraeffii sp. nov. did not produce domoic acid (DA) and were not sexually compatible with any other Pseudo-nitzschia clones tested. A total of 18 Pseudo-nitzschia species, including three confirmed toxigenic species (P. cuspidata, P. multistriata and P. australis) have now been unequivocally confirmed from eastern Australia. 
PY 2018
PD APR
SO Plos One
SN 1932-6203
PU Public Library Science
VL 13
IS 4
UT 000429791900062
DI 10.1371/journal.pone.0195622
ID 54794
ER

EF