FN Archimer Export Format
PT J
TI Physical and functional interplay between PCNA DNA clamp and Mre11–Rad50 complex from the archaeon Pyrococcus furiosus
BT 
AF HOGREL, Gaelle
   LU, Yang
   LAURENT, Sebastien
   HENRY, Etienne
   ETIENNE, Clarisse
   DULERMO, Remi
   BOSSE, Audrey
   PLUCHON, Pierre Francois
   CLOUET-D'ORVAL, Beatrice
   FLAMENT, Didier
AS 1:1,2,3;2:1,2,3;3:1,2,3;4:1,2,3;5:4;6:4;7:1,2,3;8:1,2,3;9:1,2,3;10:4;11:1,2,3;
FF 1:PDG-REM-EEP-LMEE;2:PDG-REM-EEP-LMEE;3:PDG-REM-EEP-LMEE;4:;5:;6:;7:PDG-REM-EEP-LMEE;8:PDG-REM-EEP-LMEE;9:;10:;11:PDG-REM-EEP-LMEE;
C1 IFREMER, UMR6197, Lab Microbiol Environm Extremes, F-29280 Plouzane, France.
   Univ Bretagne Occidentale, UMR6197, Lab Microbiol Environm Extremes, F-29280 Plouzane, France.
   CNRS, UMR6197, Lab Microbiol Environm Extremes, F-29280 Plouzane, France.
   Univ Toulouse, 118 Route Narbonne, F-31062 Toulouse, France.
C2 IFREMER, FRANCE
   UBO, FRANCE
   CNRS, FRANCE
   UNIV TOULOUSE, FRANCE
SI BREST
SE PDG-REM-EEP-LMEE
UM BEEP-LM2E
IN WOS Ifremer jusqu'en 2018
   DOAJ
   copubli-france
   copubli-univ-france
IF 11.147
TC 5
UR https://archimer.ifremer.fr/doc/00439/55092/56534.pdf
   https://archimer.ifremer.fr/doc/00439/55092/56535.pdf
LA English
DT Article
AB Several archaeal species prevalent in extreme environments are particularly exposed to factors likely to cause DNA damages. These include hyperthermophilic archaea (HA), living at temperatures >70°C, which arguably have efficient strategies and robust genome guardians to repair DNA damage threatening their genome integrity. In contrast to Eukarya and other archaea, homologous recombination appears to be a vital pathway in HA, and the Mre11–Rad50 complex exerts a broad influence on the initiation of this DNA damage response process. In a previous study, we identified a physical association between the Proliferating Cell Nuclear Antigen (PCNA) and the Mre11–Rad50 (MR) complex. Here, by performing co-immunoprecipitation and SPR analyses, we identified a short motif in the C- terminal portion of Pyrococcus furiosus Mre11 involved in the interaction with PCNA. Through this work, we revealed a PCNA-interaction motif corresponding to a variation on the PIP motif theme which is conserved among Mre11 sequences of Thermococcale species. Additionally, we demonstrated functional interplay in vitro between P. furiosus PCNA and MR enzymatic functions in the DNA end resection process. At physiological ionic strength, PCNA stimulates MR nuclease activities for DNA end resection and promotes an endonucleolytic incision proximal to the 5′ strand of double strand DNA break. 
PY 2018
PD JUL
SO Nucleic Acids Research
SN 0305-1048
PU Oxford Univ Press
VL 46
IS 11
UT 000438362400026
BP 5651
EP 5663
DI 10.1093/nar/gky322
ID 55092
ER

EF