Tissue Distribution and Elimination of Ciguatoxins in Tridacna maxima (Tridacnidae, Bivalvia) Fed Gambierdiscus polynesiensis

Type Article
Date 2018-05
Language English
Author(s) Roue MelanieORCID1, Darius Helene Taiana2, Ung Andre2, Viallon Jerome2, Sibat ManoellaORCID3, Hess PhilippORCID3, Amzil Zouher3, Chinain Mireille2
Affiliation(s) 1 : IRD, UMR EIO 241, POB 53267, F-98716 Tahiti, French Polynesi, France.
2 : ILM, Lab Tox Microalgae, UMR EIO 241, POB 30, F-98713 Tahiti, French Polynesi, France.
3 : IFREMER, Phycotoxins Lab, F-44311 Nantes, France.
Source Toxins (2072-6651) (Mdpi), 2018-05 , Vol. 10 , N. 5 , P. 189 (13p.)
DOI 10.3390/toxins10050189
WOS© Times Cited 4
Keyword(s) giant clams, ex situ exposure to toxic algae, ciguatoxins, Gambierdiscus polynesiensis, anatomical distribution, toxin elimination, CBA-N2a, LC-MS/MS
Abstract

Ciguatera is a foodborne disease caused by the consumption of seafood contaminated with ciguatoxins (CTXs). Ciguatera-like poisoning events involving giant clams (Tridacna maxima) are reported occasionally from Pacific islands communities. The present study aimed at providing insights into CTXs tissue distribution and detoxification rate in giant clams exposed to toxic cells of Gambierdiscus polynesiensis, in the framework of seafood safety assessment. In a first experiment, three groups of tissue (viscera, flesh and mantle) were dissected from exposed individuals, and analyzed for their toxicity using the neuroblastoma cell-based assay (CBA-N2a) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses. The viscera, flesh, and mantle were shown to retain 65%, 25%, and 10% of the total toxin burden, respectively. All tissues reached levels above the safety limit recommended for human consumption, suggesting that evisceration alone, a practice widely used among local populations, is not enough to ensure seafood safety. In a second experiment, the toxin content in contaminated giant clams was followed at different time points (0, 2, 4, and 6 days post-exposure). Observations suggest that no toxin elimination is visible in T. maxima throughout 6 days of detoxification.

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Roue Melanie, Darius Helene Taiana, Ung Andre, Viallon Jerome, Sibat Manoella, Hess Philipp, Amzil Zouher, Chinain Mireille (2018). Tissue Distribution and Elimination of Ciguatoxins in Tridacna maxima (Tridacnidae, Bivalvia) Fed Gambierdiscus polynesiensis. Toxins, 10(5), 189 (13p.). Publisher's official version : https://doi.org/10.3390/toxins10050189 , Open Access version : https://archimer.ifremer.fr/doc/00440/55123/