FN Archimer Export Format
PT J
TI Tissue Distribution and Elimination of Ciguatoxins in Tridacna maxima (Tridacnidae, Bivalvia) Fed Gambierdiscus polynesiensis
BT 
AF ROUE, Melanie
   DARIUS, Helene Taiana
   UNG, Andre
   VIALLON, Jerome
   SIBAT, Manoella
   HESS, Philipp
   AMZIL, Zouher
   CHINAIN, Mireille
AS 1:1;2:2;3:2;4:2;5:3;6:3;7:3;8:2;
FF 1:;2:;3:;4:;5:PDG-ODE-DYNECO-PHYC;6:PDG-ODE-DYNECO-PHYC;7:PDG-ODE-DYNECO-PHYC;8:;
C1 IRD, UMR EIO 241, POB 53267, F-98716 Tahiti, French Polynesi, France.
   ILM, Lab Tox Microalgae, UMR EIO 241, POB 30, F-98713 Tahiti, French Polynesi, France.
   IFREMER, Phycotoxins Lab, F-44311 Nantes, France.
C2 IRD, FRANCE
   INST LOUIS MALARDE, FRANCE
   IFREMER, FRANCE
SI NANTES
SE PDG-ODE-DYNECO-PHYC
UM EIO
IN WOS Ifremer jusqu'en 2018
   DOAJ
   copubli-france
   copubli-p187
IF 3.895
TC 14
UR https://archimer.ifremer.fr/doc/00440/55123/56579.pdf
LA English
DT Article
DE ;giant clams;ex situ exposure to toxic algae;ciguatoxins;Gambierdiscus polynesiensis;anatomical distribution;toxin elimination;CBA-N2a;LC-MS/MS
AB Ciguatera is a foodborne disease caused by the consumption of seafood contaminated with ciguatoxins (CTXs). Ciguatera-like poisoning events involving giant clams (Tridacna maxima) are reported occasionally from Pacific islands communities. The present study aimed at providing insights into CTXs tissue distribution and detoxification rate in giant clams exposed to toxic cells of Gambierdiscus polynesiensis, in the framework of seafood safety assessment. In a first experiment, three groups of tissue (viscera, flesh and mantle) were dissected from exposed individuals, and analyzed for their toxicity using the neuroblastoma cell-based assay (CBA-N2a) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses. The viscera, flesh, and mantle were shown to retain 65%, 25%, and 10% of the total toxin burden, respectively. All tissues reached levels above the safety limit recommended for human consumption, suggesting that evisceration alone, a practice widely used among local populations, is not enough to ensure seafood safety. In a second experiment, the toxin content in contaminated giant clams was followed at different time points (0, 2, 4, and 6 days post-exposure). Observations suggest that no toxin elimination is visible in T. maxima throughout 6 days of detoxification. 
PY 2018
PD MAY
SO Toxins
SN 2072-6651
PU Mdpi
VL 10
IS 5
UT 000435198800020
DI 10.3390/toxins10050189
ID 55123
ER

EF