FN Archimer Export Format
PT J
TI Cellular responses of Pacific oyster ( Crassostrea gigas ) gametes exposed in vitro to polystyrene nanoparticles
BT 
AF GONZALEZ-FERNANDEZ, Carmen
   TALLEC, Kevin
   LE GOIC, Nelly
   LAMBERT, Christophe
   SOUDANT, Philippe
   HUVET, Arnaud
   SUQUET, Marc
   BERCHEL, Mathieu
   PAUL-PONT, Ika
AS 1:1;2:2;3:1;4:1;5:1;6:2;7:2;8:3;9:1;
FF 1:;2:PDG-RBE-PFOM;3:;4:;5:;6:PDG-RBE-PFOM-LPI;7:PDG-RBE-PFOM-LPI;8:;9:;
C1 IUEM, Lab Sci Environm Marin LEMAR, UMR CNRS 6539, UBO,IRD,IFREMER, Rue Dumont Urville, F-29280 Plouzane, France.
   IFREMER, Lab Sci Environm Marin LEMAR, CS 10070, F-29280 Plouzane, France.
   Univ Bretagne Occidentale, UFR Sci, CEMCA, UMR CNRS 6521,IBSAM, 6 Ave Victor Le Gorgeu, F-29238 Brest, France.
C2 CNRS, FRANCE
   IFREMER, FRANCE
   UBO, FRANCE
SI BREST
   ARGENTON
SE PDG-RBE-PFOM
   PDG-RBE-PFOM-LPI
UM LEMAR
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 5.108
TC 92
UR https://archimer.ifremer.fr/doc/00443/55459/56976.pdf
LA English
DT Article
DE ;Nanoplastics;Oysters;Gametes;Cellular responses
AB While the detection and quantification of nano-sized plastic in the environment remains a challenge, the growing number of polymer applications mean that we can expect an increase in the release of nanoplastics into the environment by indirect outputs. Today, very little is known about the impact of nano-sized plastics on marine organisms. Thus, the objective of this study was to investigate the toxicity of polystyrene nanoplastics (NPs) on oyster (Crassostrea gigas) gametes. Spermatozoa and oocytes were exposed to four NPs concentrations ranging from 0.1 to 100 mg L−1 for 1, 3 and 5 h. NPs coated with carboxylic (PS-COOH) and amine groups (PS-NH2) were used to determine how surface properties influence the effects of nanoplastics. Results demonstrated the adhesion of NPs to oyster spermatozoa and oocytes as suggested by the increase of relative cell size and complexity measured by flow-cytometry and confirmed by microscopy observations. A significant increase of ROS production was observed in sperm cells upon exposure to 100 mg L−1 PS-COOH, but was not observed with PS-NH2, suggesting a differential effect according to the NP-associated functional group. Altogether, these results demonstrate that the effects of NPs occur rapidly, are complex and are possibly associated with the cellular eco-corona, which could modify NPs behaviour and toxicity. 
PY 2018
PD OCT
SO Chemosphere
SN 0045-6535
PU Pergamon-elsevier Science Ltd
VL 208
UT 000441999400085
BP 764
EP 772
DI 10.1016/j.chemosphere.2018.06.039
ID 55459
ER

EF