Optimal age of the donor graft tissue in relation to cultured pearl phenotypes in the mollusc, Pinctada margaritifera

Type Article
Date 2018-06
Language English
Author(s) Blay Carole1, 2, Planes Serge2, Ky Chin-Long1
Affiliation(s) 1 : IFREMER, UMR 241, Ctr Pacifique, EIO,Labex Corail, Tahiti, French Polynesi, France.
2 : PSL Res Univ, USR 3278, Univ Perpignan, EPHE,UPVD,CNRS,CRIOBE,Labex Corail, Perpignan, France.
Source Plos One (1932-6203) (Public Library Science), 2018-06 , Vol. 13 , N. 6 , P. e0198505 (14p.)
DOI 10.1371/journal.pone.0198505
WOS© Times Cited 4
Abstract

Ageing is defined as the progressive decline in tissue and organ functions over time. This study aims to evaluate the ageing effect on cultured pearl quality phenotypes (including size and quality traits) in the graft-recipient animal model: Pinctada margaritifera. For this, eight uniform grafting experiments were designed using two hatchery-produced pearl oyster families as donors, which were followed through time, between 7 and 30 months in age. For each age category, 20 donors were studied for each culture site giving a total of 2400 grafted oysters. Several phenotypic measurements were made: 1) donor family growth performance from shell size records, 2) pearl size and corresponding quality traits, and 3) expression of some genes related to biomineralization processes on both the mantle graft and on pearl sac tissues. Results showed that: 1) donor age has an impact on pearl size, with grafts coming from the youngest donors yielding the biggest pearls; and 2) grafts from donors between 12 and 18 months in age produced pearls of the highest quality (grade and surface quality), a result supported by an analysis where the level of expression for a panel of genes associated with biomineralization was greatest in donors within the 12 to 18 months age group. These results indicate that donors aged between 12 and 18 months have high potential for biomineralisation and nacre deposition, and likely produce larger and higher quality cultured pearls than older donors.

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Publisher's official version 14 4 MB Open access
S1 Table. Set of forward and reverse primers used for the biomineralization gene expression analysis in Pinctada margaritifera. 1 72 KB Open access
S1 Appendix. Relative stability of the GAPDH and SAGE genes combination confirmed using NormFinder (Stability value for best combination). 1 5 KB Open access
S2 Appendix. All quantitative and qualitative figures datas. 134 KB Open access
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