FN Archimer Export Format
PT J
TI Zeaxanthin from Porphyridium purpureum induces apoptosis in human melanoma cells expressing the oncogenic BRAF V600E mutation and sensitizes them to the BRAF inhibitor vemurafenib
BT 
AF JUIN, Camille
   DE OLIVEIRA JUNIOR, Raimundo Gonsalves
   FLEURY, Audrey
   OUDINET, Chloe
   PYTOWSKI, Lior
   BERARD, Jean-Baptiste
   NICOLAU, Elodie
   THIERY, Valerie
   LANNELUC, Isabelle
   BEAUGEARD, Laureen
   PRUNIER, Gregoire
   GUEDES DA SILVA ALMEIDA, Jackson Roberto
   PICOT, Laurent
AS 1:1;2:1,3;3:1;4:1;5:1;6:2;7:2;8:1;9:1;10:1;11:1;12:3;13:1;
FF 1:;2:;3:;4:;5:;6:PDG-RBE-BRM-LPBA;7:PDG-RBE-BRM-LPBA;8:;9:;10:;11:;12:;13:;
C1 Univ La Rochelle, CNRS, Littoral Environm & Soc UMR 7266, La Rochelle, France.
   Inst Francais Rech Exploitat Mer, Lab Physiol & Biotechnol Algues, Lab Biotechnol & Ressources Marines, Nantes, France.
   Fed Univ San Francisco Valley, Ctr Studies & Res Med Plants, Petrolina, PE, Brazil.
C2 UNIV LA ROCHELLE, FRANCE
   IFREMER, FRANCE
   FED UNIV SAN FRANCISCO VALLEY, BRAZIL
SI NANTES
SE PDG-RBE-BRM-LPBA
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
   copubli-sud
IF 1.754
TC 19
UR https://archimer.ifremer.fr/doc/00444/55595/57227.pdf
LA English
DT Article
DE ;Cancer;Carotenoid;Melanoma;Microalgae;Phytoplankton;Zeaxanthin
AB Zeaxanthin, an abundant carotenoid present in fruits, vegetables and algae was reported to exert antiproliferative activity and induce apoptosis in human uveal melanoma cells. It also inhibited uveal melanoma tumor growth and cell migration in nude mice xenograft models. Here we report that zeaxanthin purified from the rhodophyte Porphyridium purpureum (Bory) K.M.Drew & R.Ross, Porphyridiaceae, promotes apoptosis in the A2058 human melanoma cell line expressing the oncogenic BRAF V600E mutation. Zeaxanthin 40 μM (IC50) induced chromatin condensation, nuclear blebbing, hypodiploidy, accumulation of cells in sub-G1 phase, DNA internucleosomal fragmentation and activation of caspase-3. Western blot analysis revealed that zeaxanthin induced up-regulation of the pro-apoptotic factors Bim and Bid and inhibition of NF-κB transactivation. Additionally, zeaxanthin sensitized A2058 melanoma cells in vitro to the cytotoxic activity of vemurafenib, a BRAF inhibitor widely used for the clinical management of melanoma, suggesting its potential interest as dietary adjuvant increasing melanoma cells sensitivity to chemotherapy. 
PY 2018
PD JUN
SO Revista Brasileira De Farmacognosia-brazilian Journal Of Pharmacognosy
SN 0102-695X
PU Soc Brasileira Farmacognosia
VL 28
IS 4
UT 000439145100010
BP 457
EP 467
DI 10.1016/j.bjp.2018.05.009
ID 55595
ER

EF