FN Archimer Export Format
PT J
TI Genome-wide association and genomic prediction of resistance to viral nervous necrosis in European sea bass (Dicentrarchus labrax) using RAD sequencing
BT 
AF PALAIOKOSTAS, Christos
   CARIOU, Sophie
   BESTIN, Anastasia
   BRUANT, Jean-Sebastien
   HAFFRAY, Pierrick
   MORIN, Thierry
   CABON, Joelle
   ALLAL, Francois
   VANDEPUTTE, Marc
   HOUSTON, Ross D.
AS 1:1;2:2;3:3;4:2;5:3;6:4;7:4;8:5;9:6;10:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:PDG-RBE-MARBEC-LAAAS;9:;10:;
C1 Univ Edinburgh, Roslin Inst, Royal Dick Sch Vet Studies, Easter Bush EH25 9RG, Midlothian, Scotland.
   Ferme Marine Douhet, BP 4, F-17840 La Bree Les Bains, France.
   LPGP INRA, SYSAAF, Campus Beaulieu, F-35042 Rennes, France.
   Bretagne Loire Univ, Viral Fish Pathol Unit,Technopole Brest Iroise, French Agcy Food Environm & Occupat Hlth & Safety, Natl Reference Lab Regulated Fish Dis,Ploufragan, BP 70, F-29280 Plouzane, France.
   Univ Montpellier, Ifremer CNRS IRD UM, MARBEC, Palavas Les Flots, France.
   Univ Paris Saclay, GABI, INRA, AgroParisTech, F-78350 Jouy En Josas, France.
C2 UNIV EDINBURGH, UK
   FERME MARINE DOUHET, FRANCE
   INRA, FRANCE
   ANSES, FRANCE
   IFREMER, FRANCE
   INRA, FRANCE
SI PALAVAS
SE PDG-RBE-MARBEC-LAAAS
UM MARBEC
IN WOS Ifremer jusqu'en 2018
   DOAJ
   copubli-france
   copubli-p187
   copubli-europe
IF 3.094
TC 69
UR https://archimer.ifremer.fr/doc/00445/55676/57357.pdf
LA English
DT Article
AB Background: European sea bass (Dicentrarchus labrax) is one of the most important species for European aquaculture. Viral nervous necrosis (VNN), commonly caused by the redspotted grouper nervous necrosis virus (RGNNV), can result in high levels of morbidity and mortality, mainly during the larval and juvenile stages of cultured sea bass. In the absence of efficient therapeutic treatments, selective breeding for host resistance offers a promising strategy to control this disease. Our study aimed at investigating genetic resistance to VNN and genomic-based approaches to improve disease resistance by selective breeding. A population of 1538 sea bass juveniles from a factorial cross between 48 sires and 17 dams was challenged with RGNNV with mortalities and survivors being recorded and sampled for genotyping by the RAD sequencing approach. Results: We used genome-wide genotype data from 9195 single nucleotide polymorphisms (SNPs) for downstream analysis. Estimates of heritability of survival on the underlying scale for the pedigree and genomic relationship matrices were 0.27 (HPD interval 95%: 0.14-0.40) and 0.43 (0.29-0.57), respectively. Classical genome-wide association analysis detected genome-wide significant quantitative trait loci (QTL) for resistance to VNN on chromosomes (unassigned scaffolds in the case of 'chromosome' 25) 3, 20 and 25 (P < 1e06). Weighted genomic best linear unbiased predictor provided additional support for the QTL on chromosome 3 and suggested that it explained 4% of the additive genetic variation. Genomic prediction approaches were tested to investigate the potential of using genome-wide SNP data to estimate breeding values for resistance to VNN and showed that genomic prediction resulted in a 13% increase in successful classification of resistant and susceptible animals compared to pedigree-based methods, with Bayes A and Bayes B giving the highest predictive ability. Conclusions: Genome-wide significant QTL were identified but each with relatively small effects on the trait. Tests of genomic prediction suggested that incorporating genome-wide SNP data is likely to result in higher accuracy of estimated breeding values for resistance to VNN. RAD sequencing is an effective method for generating such genomewide SNPs, and our findings highlight the potential of genomic selection to breed farmed European sea bass with improved resistance to VNN. 
PY 2018
PD JUL
SO Genetics Selection Evolution
SN 0999-193X
PU Biomed Central Ltd
VL 50
IS 30
UT 000435003900001
DI 10.1186/s12711-018-0401-2
ID 55676
ER

EF