Concentration and purification of Porphyridium cruentum exopolysaccharides by membrane filtration at various cross-flow velocities

Type Article
Date 2018-11
Language English
Author(s) Balti Rafik1, 2, Le Balc'h Romain1, Brodu NicolasORCID1, 3, Gilbert Marthe1, Le Gouic Benjamin1, Le Gall Sophie4, Sinquin CorinneORCID5, Masse Anthony1
Affiliation(s) 1 : Univ Nantes, LUNAM, CNRS, GEPEA,UMR 6144, 37 Bd Univ, F-44602 St Nazaire, France.
2 : Univ Jendouba, Higher Inst Biotechnol Beja, Unite Physiol Fonct & Valorisat Bioressources UR1, PB 382,Habib Bourguiba Ave, Beja 9000, Tunisia.
3 : Normandie Univ, UNIROUEN, INSA Rouen, LSPC, F-76000 Rouen, France.
4 : INRA, BIA, UR1268, Rue Geraudiere, F-44300 Nantes, France.
5 : IFREMER, Lab EM3B, Rue Ile Yeu, F-44311 Nantes 3, France.
Source Process Biochemistry (1359-5113) (Elsevier Sci Ltd), 2018-11 , Vol. 74 , P. 175-184
DOI 10.1016/j.procbio.2018.06.021
WOS© Times Cited 33
Keyword(s) Membrane filtration, Exopolysaccharides, Microalga, Concentration, Diafiltration

Exopolysaccharides (EPS) from cell-free Porphyridium cruentum media were concentrated then purified (diafiltration) on a 0.14 µm ceramic membrane. The influence of cross-flow velocities on filtration performances was investigated. Mean permeate fluxes equal to 49.8, 68.9 and 81.9 L.h-1. m-2 were obtained during the concentration at 4 bar for respectively cross-flow velocities inside the membrane lumen equal to 2.5, 3.3 and 4.2 m.s-1; 49.7 L.h-1. m-2 for the diafiltration at 3.3 m.s-1. Permeate fluxes were correctly predicted from polysaccharide concentrations (10 % deviation). Volume reduction factors higher than 7.8 were reached. Rejection rates of polysaccharides and proteins varied according to the cross-flow velocities. Thus, the EPS recovery rate or time of filtration could be modulated following the cross-flow velocity. Polysaccharides were concentrated 6.3 to 10.4 times in such a way that the final sugars concentration reached 1.74 to 2.26 g.L-1. Rheological behavior of filtered solutions changed following the concentration progress. More than 80 % (w/w) of polysaccharides were recovered while 49 % and 99 % of proteins and salts were removed respectively. The filtrations allowed reaching a final monosaccharide content of dry matter equal to 48.9 % against 0.6 % (w/w) initially.

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Balti Rafik, Le Balc'h Romain, Brodu Nicolas, Gilbert Marthe, Le Gouic Benjamin, Le Gall Sophie, Sinquin Corinne, Masse Anthony (2018). Concentration and purification of Porphyridium cruentum exopolysaccharides by membrane filtration at various cross-flow velocities. Process Biochemistry, 74, 175-184. Publisher's official version : , Open Access version :