FN Archimer Export Format PT J TI Concentration and purification of Porphyridium cruentum exopolysaccharides by membrane filtration at various cross-flow velocities BT AF BALTI, Rafik LE BALC'H, Romain BRODU, Nicolas GILBERT, Marthe LE GOUIC, Benjamin LE GALL, Sophie SINQUIN, Corinne MASSE, Anthony AS 1:1,2;2:1;3:1,3;4:1;5:1;6:4;7:5;8:1; FF 1:;2:;3:;4:;5:;6:;7:PDG-RBE-BRM-LEMMMB;8:; C1 Univ Nantes, LUNAM, CNRS, GEPEA,UMR 6144, 37 Bd Univ, F-44602 St Nazaire, France. Univ Jendouba, Higher Inst Biotechnol Beja, Unite Physiol Fonct & Valorisat Bioressources UR1, PB 382,Habib Bourguiba Ave, Beja 9000, Tunisia. Normandie Univ, UNIROUEN, INSA Rouen, LSPC, F-76000 Rouen, France. INRA, BIA, UR1268, Rue Geraudiere, F-44300 Nantes, France. IFREMER, Lab EM3B, Rue Ile Yeu, F-44311 Nantes 3, France. C2 UNIV NANTES, FRANCE UNIV JENDOUBA, TUNISIA UNIV NORMANDIE, FRANCE INRA, FRANCE IFREMER, FRANCE SI NANTES SE PDG-RBE-BRM-LEMMMB IN WOS Ifremer jusqu'en 2018 copubli-france copubli-p187 copubli-univ-france copubli-int-hors-europe copubli-sud IF 2.883 TC 35 UR https://archimer.ifremer.fr/doc/00445/55694/57360.pdf LA English DT Article DE ;Membrane filtration;Exopolysaccharides;Microalga;Concentration;Diafiltration AB Exopolysaccharides (EPS) from cell-free Porphyridium cruentum media were concentrated then purified (diafiltration) on a 0.14 µm ceramic membrane. The influence of cross-flow velocities on filtration performances was investigated. Mean permeate fluxes equal to 49.8, 68.9 and 81.9 L.h-1. m-2 were obtained during the concentration at 4 bar for respectively cross-flow velocities inside the membrane lumen equal to 2.5, 3.3 and 4.2 m.s-1; 49.7 L.h-1. m-2 for the diafiltration at 3.3 m.s-1. Permeate fluxes were correctly predicted from polysaccharide concentrations (10 % deviation). Volume reduction factors higher than 7.8 were reached. Rejection rates of polysaccharides and proteins varied according to the cross-flow velocities. Thus, the EPS recovery rate or time of filtration could be modulated following the cross-flow velocity. Polysaccharides were concentrated 6.3 to 10.4 times in such a way that the final sugars concentration reached 1.74 to 2.26 g.L-1. Rheological behavior of filtered solutions changed following the concentration progress. More than 80 % (w/w) of polysaccharides were recovered while 49 % and 99 % of proteins and salts were removed respectively. The filtrations allowed reaching a final monosaccharide content of dry matter equal to 48.9 % against 0.6 % (w/w) initially. PY 2018 PD NOV SO Process Biochemistry SN 1359-5113 PU Elsevier Sci Ltd VL 74 UT 000447577800022 BP 175 EP 184 DI 10.1016/j.procbio.2018.06.021 ID 55694 ER EF