Ecologically realistic model of infection for exploring the host damage caused by Vibrio aestuarianus

Type Article
Date 2018-12
Language English
Author(s) Parizadeh Leila1, Tourbiez Delphine1, Garcia Celine1, Haffner Philippe1, 2, Degremont LionelORCID1, Le Roux Frederique3, 4, Travers Marie-AgnesORCID1
Affiliation(s) 1 : IFREMER, SG2M LGPMM, Lab Genet & Pathol Mollusques Marins, Ave Mus Loup, F-17390 La Tremblade, France.
2 : UM, IFREMER, CNRS, IHPE,UPVD,UMR 5244, Pl Eugene Bataillon CC80, F-34095 Montpellier 05, France.
3 : IFREMER, Unite Physiol Fonct Organismes Marins, CS 10070, F-29280 Plouzane, France.
4 : UPMC Paris 06, Sorbonne Univ, Stn Biol Roscoff, Integrat Biol Marine Models,CNRS,UMR 8227, CS 90074, F-29688 Roscoff, France.
Source Environmental Microbiology (1462-2912) (Wiley), 2018-12 , Vol. 20 , N. 12 , P. 4343-4355
DOI 10.1111/1462-2920.14350
WOS© Times Cited 4
Note Special Issue: EMI is 20! Part 2
Abstract

Although vibrios are frequently associated with marine organisms mortality outbreaks, knowledge on their ecology and pathogenicity is sparse, thus limiting disease management and prophylactic strategies. Here, we investigated V. aestuarianus infection onset and progression in the wild, taking advantage of a “claire” pond: a semi‐closed system with limited seawater renewal, theoretically more adapted to disease transmission. We showed a positive association of the bacteria with oysters, which can constitute a reservoir for the bacteria in the winter. Moreover, passage through oysters was found to be necessary for experimental disease reproduction as vibrios shedding from diseased oysters has higher infectivity than from in vitro grown. We next developed an experimental “ecologically realistic” infection model in a mesocosm, allowing infection by natural route. By means of this non‐invasive protocol, we analysed the pathogenesis of the bacteria and demonstrated the importance of haemolymph for initial colonisation and the septicaemic nature of this disease.

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Parizadeh Leila, Tourbiez Delphine, Garcia Celine, Haffner Philippe, Degremont Lionel, Le Roux Frederique, Travers Marie-Agnes (2018). Ecologically realistic model of infection for exploring the host damage caused by Vibrio aestuarianus. Environmental Microbiology, 20(12), 4343-4355. Publisher's official version : https://doi.org/10.1111/1462-2920.14350 , Open Access version : https://archimer.ifremer.fr/doc/00446/55761/