FN Archimer Export Format
PT J
TI The RCR and ATP/O indices can give contradictory messages about mitochondrial efficiency
BT 
AF SALIN, Karine
   VILLASEVIL, Eugenia M.
   ANDERSON, Graeme J.
   SELMAN, Colin
   CHINOPOULOS, Christos
   METCALFE, Neil B.
AS 1:1,2;2:1;3:1;4:1;5:3,4;6:1;
FF 1:PDG-RBE-PFOM-LARN;2:;3:;4:;5:;6:;
C1 Univ Glasgow, Inst Biodivers Anim Hlth & Comparat Med, Graham Kerr Bldg, Glasgow G12 8QQ, Lanark, Scotland.
   IFREMER, Unite Physiol Fonct Organismes Marins LEMAR UMR 6, BP70, F-29280 Plouzane, France.
   Semmelweis Univ, Dept Med Biochem, H-1094 Budapest, Hungary.
   MTA SE Lendulet Neurobiochem Res Grp, H-1094 Budapest, Hungary.
C2 UNIV GLASGOW, UK
   IFREMER, FRANCE
   UNIV SEMMELWEIS, HUNGARY
   UNIV SEMMELWEIS, HUNGARY
SI BREST
SE PDG-RBE-PFOM-LARN
UM LEMAR
IN WOS Ifremer jusqu'en 2018
   copubli-europe
IF 3.101
TC 21
UR https://archimer.ifremer.fr/doc/00449/56014/57529.pdf
LA English
DT Article
AB Mitochondrial efficiency is typically taken to represent an animal’s capacity to convert its resources into ATP. However, the term mitochondrial efficiency, as currently used in the literature, can be calculated as either the respiratory control ratio, RCR (ratio of mitochondrial respiration supporting ATP synthesis to that required to offset the proton leak) or as the amount of ATP generated per unit of oxygen consumed, ATP/O ratio. The question of how flexibility in mitochondrial energy properties (i.e. in rates of respiration to support ATP synthesis and offset proton leak, and in the rate of ATP synthesis) affects these indices of mitochondrial efficiency has tended to be overlooked. Furthermore, little is known of whether the RCR and ATP/O ratio vary in parallel, either among individuals or in response to environmental conditions. Using data from brown trout Salmo trutta we show that experimental conditions affect mitochondrial efficiency, but the apparent direction of change depends on the index chosen: a reduction in food availability was associated with an increased RCR (i.e. increased efficiency) but a decreased ATP/O ratio (decreased efficiency) in liver mitochondria. Moreover, there was a negative correlation across individuals held in identical conditions between their RCR and their ATP/O ratio. These results show that the choice of index of mitochondrial efficiency can produce different, even opposing, conclusions about the capacity of the mitochondria to produce ATP. Neither ratio is necessarily a complete measure of efficiency of ATP production in the living animal (RCR because it contains no assessment of ATP production, and ATP/O because it contains no assessment of respiration to offset the proton leak). Consequently, we suggest that a measure of mitochondrial efficiency obtained nearer to conditions where respiration simultaneously offsets the proton leak and produce ATP would be sensitive to changes in both proton leakage and ATP production, and is thus likely to be more representative of the state of the mitochondria in vivo. 
PY 2018
PD SEP
SO Integrative And Comparative Biology
SN 1540-7063
PU Oxford Univ Press Inc
VL 58
IS 3
UT 000451996200012
BP 486
EP 494
DI 10.1093/icb/icy085
ID 56014
ER

EF