FN Archimer Export Format
PT J
TI Bioprotective Effect of Lactococcus piscium CNCM I-4031 Against Listeria monocytogenes Growth and Virulence
BT 
AF SARAOUI, Taous
   LEROI, Francoise
   CHEVALIER, Frederique
   CAPPELIER, Jean-Michel
   PASSERINI, Delphine
   PILET, Marie-France
AS 1:1,2;2:1;3:1;4:2;5:1;6:2;
FF 1:;2:PDG-RBE-BRM-LEMMMB;3:PDG-RBE-BRM-LEMMMB;4:;5:PDG-RBE-BRM-LEMMMB;6:;
C1 Inst Francais Rech Exploitat Mer Ifremer, Lab Ecosyst Microbiens & Mol Marines Biotechnol E, Nantes, France.
   Ecole Natl Vet Agroalimentaire & Alimentat Nantes, INRA, SECALIM UMR1014, Nantes, France.
C2 IFREMER, FRANCE
   INRA, FRANCE
SI NANTES
SE PDG-RBE-BRM-LEMMMB
IN WOS Ifremer jusqu'en 2018
   DOAJ
   copubli-france
   copubli-p187
IF 4.259
TC 18
UR https://archimer.ifremer.fr/doc/00449/56065/57588.pdf
LA English
DT Article
DE ;biopreservation;Lactococcus piscium;Listeria monocytogenes;co-culture;cell ratio;scanning electron microscopy;virulence
AB Listeria monocytogenes is a Gram-positive pathogen occurring in many refrigerated ready-to-eat foods. It is responsible for foodborne listeriosis, a rare but severe disease with a high mortality rate (20–30%). Lactococcus piscium CNCM I-4031 has the capacity to prevent the growth of L. monocytogenes in contaminated peeled and cooked shrimp and in a chemically defined medium using a cell-to-cell contact-dependent mechanism. To characterize this inhibition further, the effect of L. piscium was tested on a collection of 42 L. monocytogenes strains. All strains were inhibited but had different sensitivities. The effect of the initial concentration of the protective and the target bacteria revealed that the inhibition always occurred when L. piscium had reached its maximum population density, whatever the initial concentration of the protective bacteria. Viewed by scanning electron microscopy, L. monocytogenes cell shape and surface appeared modified in co-culture with L. piscium CNCM I-4031. Lastly, L. monocytogenes virulence, evaluated by a plaque-forming assay on the HT-29 cell line, was reduced after cell pre-treatment by the protective bacteria. In conclusion, the bioprotective effect of L. piscium toward L. monocytogenes growth and virulence was demonstrated, and a hypothesis for the inhibition mechanism is put forward. 
PY 2018
PD JUN
SO Frontiers In Microbiology
SN 1664-302X
PU Frontiers Media Sa
VL 9
IS 1564
UT 000438921600001
DI 10.3389/fmicb.2018.01564
ID 56065
ER

EF