FN Archimer Export Format
PT J
TI Microplastic bacterial communities in the Bay of Brest: Influence of polymer type and size
BT 
AF FRERE, Laura
   MAIGNIEN, Lois
   CHALOPIN, Morgane
   HUVET, Arnaud
   RINNERT, Emmanuel
   MORRISON, Hilary
   KERNINON, Sandrine
   CASSONE, Anne-Laure
   LAMBERT, Christophe
   REVEILLAUD, Julie
   PAUL-PONT, Ika
AS 1:1;2:2;3:3;4:3;5:4;6:5;7:6;8:1;9:1;10:7;11:1;
FF 1:;2:;3:;4:PDG-RBE-PFOM-LPI;5:PDG-REM-GM-LCG;6:;7:;8:;9:;10:PDG-REM-EEP-LMEE;11:;
C1 Inst Univ Europeen Mer, Lab Sci Environm Marin LEMAR, IFREMER, UMR 6539,CNRS,UBO,IRD, Technopole Brest Iroise,Rue Dumont dUrville, F-29280 Plouzane, France.
   Inst Univ Europeen Mer, Lab Microbiol Environm Extremes, IFREMER, UMR 6197,UBO,CNRS, Technopole Brest Iroise,Rue Dumont dUrville, F-29280 Plouzane, France.
   IFREMER, Lab Sci Environm Marin LEMAR, UMR 6539, UBO,CNRS,IRD,Ctr Bretagne, CS 10070, F-29280 Plouzane, France.
   IFREMER, Lab Detect Capteurs Mesures, RDT LDCM, Ctr Bretagne, ZI Pointe Diable,CS 10070, F-29280 Plouzane, France.
   Josephine Bay Paul Ctr Mol Biol & Evolut, Marine Biol Lab, 7 MBL St, Woods Hole, MA USA.
   LABOCEA, 22 Ave Plage Gueux,CS 13031, F-29334 Quimper, France.
   Univ Montpellier, INRA, CIRAD, ASTRE, Montpellier, France.
C2 UBO, FRANCE
   UBO, FRANCE
   IFREMER, FRANCE
   IFREMER, FRANCE
   MARINE BIOLOGICAL LAB, USA
   LABOCEA, FRANCE
   UNIV MONTPELLIER, FRANCE
SI BREST
SE PDG-RBE-PFOM-LPI
   PDG-REM-GM-LCG
   PDG-REM-EEP-LMEE
UM BEEP-LM2E
   LEMAR
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
IF 5.714
TC 236
UR https://archimer.ifremer.fr/doc/00449/56082/57614.pdf
LA English
DT Article
DE ;Bacteria;Microplastics;Coastal ecosystem;Metabarcoding;Vibrios
AB Microplastics (<5 mm) exhibit intrinsic features such as density, hydrophobic surface, or high surface/volume ratio, that are known to promote microbial colonization and biofilm formation in marine ecosystems. Yet, a relatively low number of studies have investigated the nature of microplastic associated bacterial communities in coastal ecosystems and the potential factors influencing their composition and structure. Here, we characterized microplastics collected in the Bay of Brest by manual sorting followed by Raman spectroscopy and studied their associated bacterial assemblages using 16S amplicon high-throughput sequencing. Our methodology allowed discriminating polymer type (polyethylene, polypropylene and polystyrene) within small size ranges (0.3–1 vs. 1–2 vs. 2–5 mm) of microplastics collected. Data showed high species richness and diversity on microplastics compared to surrounding seawater samples encompassing both free living and particle attached bacteria. Even though a high proportion of operational taxonomic units (OTU; 94 ± 4%) was shared among all plastic polymers, polystyrene fragments exhibited distinct bacterial assemblages as compared to polyethylene and polypropylene samples. No effect of microplastic size was revealed regardless of polymer type, site and date of collection. The Vibrio genus was commonly detected in the microplastic fraction and specific PCR were performed to determine the presence of potentially pathogenic Vibrio strains (namely V. aestuarianus and the V. splendidus polyphyletic group). V. splendidus related species harboring putative oyster pathogens were detected on most microplastic pools (77%) emphasizing the need of further research to understand the role of microplastics on pathogen population transport and ultimate disease emergence. 
PY 2018
PD NOV
SO Environmental Pollution
SN 0269-7491
PU Elsevier Sci Ltd
VL 242
IS Part A
UT 000446150300066
BP 614
EP 625
DI 10.1016/j.envpol.2018.07.023
ID 56082
ER

EF