FN Archimer Export Format PT J TI Pre-purification by membrane filtration of paralytic shellfish toxins from Alexandrium minutum dinoflagellate BT AF BALTI, Rafik BRODU, Nicolas ZHANG, Jiaxuan AMZIL, Zouher DROUIN, Delphine SECHET, Veronique MASSE, Anthony AS 1:1,2;2:1,3;3:1;4:4;5:1;6:4;7:1; FF 1:;2:;3:;4:PDG-ODE-DYNECO-PHYC;5:;6:PDG-ODE-DYNECO-PHYC;7:; C1 Univ Nantes, LUNAM, GEPEA, UMR CNRS 6144, 37 Bd Univ,BP 406, F-44602 St Nazaire, France. Univ Jendouba, Higher Inst Biotechnol Beja, Unite Physiol Fonct & Valorisat Bioressources UR1, PB 382,Habib Bourguiba Ave, Beja 9000, Tunisia. NormandieUniv, UNIROUEN, INSA Rouen, LSPC, F-76000 Rouen, France. IFREMER, Lab Phycotoxines, Ctr Nantes, BP 21105, F-44311 Nantes, France. C2 UNIV NANTES, FRANCE UNIV JENDOUBA, TUNISIA UNIV NORMANDIE, FRANCE IFREMER, FRANCE SI NANTES SE PDG-ODE-DYNECO-PHYC IN WOS Ifremer UPR copubli-france copubli-univ-france copubli-int-hors-europe copubli-sud IF 5.774 TC 4 UR https://archimer.ifremer.fr/doc/00451/56286/57865.pdf LA English DT Article DE ;Paralytic shellfish poisoning;Nanofiltration;Membrane;Alexandrium minutum;Purification AB The Paralytic shellfish neurotoxins (PST) are of increasing interest for biomedical applications. The chemical synthesis is often complex and expensive that’s why the purification by membrane filtration of PST from Alexandrium minutum dinoflagellate was investigated. Disrupted micro-alga cells by ultrasonic treatment were diafiltred to let pass toxins through an ultrafiltration membrane. Then, the mean permeate was concentrated and diafiltrated by nanofiltration. Mean permeate fluxes equal to 187, 135 and 135 L.h–1.m-2 were obtained during the first diafiltration, the concentration step and the final diafiltration respectively. Up to 57 % (mol/mol) and 78 % (mol/mol) of organic matters and salts were removed respectively. Divalent ions were sparsely removed contrary to monovalent ones. C1 and C2 toxins were successfully purified since more than 75 % (mol/mol) were recovered. However, only 27 to 50 % (mol/mol) of GTX2, GTX3 and STX were recovered. PY 2019 PD FEB SO Separation And Purification Technology SN 1383-5866 PU Elsevier Science Bv VL 210 UT 000449132900017 BP 152 EP 158 DI 10.1016/j.seppur.2018.07.063 ID 56286 ER EF