FN Archimer Export Format
PT J
TI A rapid quantitative fluorescence-based bioassay to study allelochemical interactions from Alexandrium minutum
BT 
AF LONG, Marc
   TALLEC, Kevin
   SOUDANT, Philippe
   LAMBERT, Christophe
   LE GRAND, Fabienne
   SARTHOU, Geraldine
   JOLLEY, Dianne
   HEGARET, Helene
AS 1:1,2;2:3;3:1;4:1;5:1;6:1;7:2;8:4;
FF 1:;2:PDG-RBE-PFOM;3:;4:;5:;6:;7:;8:;
C1 Inst Univ Europeen Mer, Lab Sci Environm Marin LEMAR, UMR CNRS UBO IRD IFREMER 6539, Technopole Brest Iroise,Rue Dumont dUrville, F-29280 Plouzane, France.
   Univ Wollongong, Sch Chem, Wollongong, NSW 2522, Australia.
   Ifremer, Lab Sci Environm Marin LEMAR, UMR UBO 6539, CNRS,IRD, F-29280 Plouzane, France.
C2 UBO, FRANCE
   UNIV WOLLONGONG, AUSTRALIA
   IFREMER, FRANCE
   CNRS, FRANCE
SI BREST
SE PDG-RBE-PFOM
UM LEMAR
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
IF 5.714
TC 20
UR https://archimer.ifremer.fr/doc/00453/56424/58116.pdf
LA English
DT Article
DE ;Harmful algal bloom;Alexandrium;Allelopathy;Cytotoxicity;PAM fluorometry;Bioassay
AB Harmful microalgal blooms are a threat to aquatic organisms, ecosystems and human health. Toxic dinoflagellates of the genus Alexandrium are known to produce paralytic shellfish toxins and to release bioactive extracellular compounds (BECs) with potent cytotoxic, hemolytic, ichtyotoxic and allelopathic activity. Negative allelochemical interactions refer to the chemicals that are released by the genus Alexandrium and that induce adverse effects on the physiology of co-occurring protists and predators. Releasing BECs gives the donor a competitive advantage that may help to form dense toxic blooms of phytoplankton. However BECs released by Alexandrium minutum are uncharacterized and it is impossible to quantify them using classical chemical methods. Allelochemical interactions are usually quantified through population growth inhibition or lytic-activity based bioassays using a secondary target organism. However these bioassays require time (for growth or microalgal counts) and/or are based on lethal effects. The use of pulse amplitude modulation (PAM) fluorometry has been widely used to assess the impact of environmental stressors on phytoplankton but rarely for allelochemical interactions. Here we evaluated the use of PAM and propose a rapid chlorophyll fluorescence based bioassay to quantify allelochemical BECs released from Alexandrium minutum. We used the ubiquitous diatom Chaetoceros muelleri as a target species. The bioassay, based on sub-lethal effects, quantifies allelochemical activity from different samples (filtrates, extracts in seawater) within a short period of time (2 h). This rapid bioassay will help investigate the role of allelochemical interactions in Alexandrium bloom establishment. It will also further our understanding of the potential relationship between allelochemical activities and other cytotoxic activities from BECs. While this bioassay was developed for the species A. minutum, it may be applicable to other species producing allelochemicals and may provide further insights into the role and impact of allelochemical interactions in forming dense algal blooms and structuring marine ecosystems. 
PY 2018
PD NOV
SO Environmental Pollution
SN 0269-7491
PU Elsevier Sci Ltd
VL 242
IS Part B
UT 000446282600060
BP 1598
EP 1605
DI 10.1016/j.envpol.2018.07.119
ID 56424
ER

EF