FN Archimer Export Format
PT J
TI High-Throughput Identification of Candidate Strains for Biopreservation by Using Bioluminescent Listeria monocytogenes
BT 
AF EL KHEIR, Sara M.
   CHERRAT, Lamia
   AWUSSI, Ahoefa A.
   RAMIA, Nancy E.
   TAHA, Samir
   RAHMAN, Abdur
   PASSERINI, Delphine
   LEROI, Francoise
   PETIT, Jeremy
   MANGAVEL, Cecile
   REVOL-JUNELLES, Anne-Marie
   BORGES, Frederic
AS 1:1,2;2:1;3:1;4:1,2;5:2;6:3;7:4;8:4;9:1;10:1;11:1;12:1;
FF 1:;2:;3:;4:;5:;6:;7:PDG-RBE-BRM-LEMMMB;8:PDG-RBE-BRM-LEMMMB;9:;10:;11:;12:;
C1 Univ Lorraine, LIBio, Nancy, France.
   Univ Libanaise, Lab Blotechnol Appl, EDST, Tripoli, Lebanon.
   Natl Univ Sci & Technol, Dept Ind Biotechnol, Atta Ur Rahman Sch Appl Biosci, Islamabad, Pakistan.
   Ifremer, Lab Ecosyst Microbiens & Mol Marines Biotechnol, Nantes, France.
C2 UNIV LORRAINE, FRANCE
   UNIV LIBANAISE, LEBANON
   UNIV NATL PAKISTAN (NUST), PAKISTAN
   IFREMER, FRANCE
SI NANTES
SE PDG-RBE-BRM-LEMMMB
IN WOS Ifremer jusqu'en 2018
   DOAJ
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
   copubli-sud
IF 4.259
TC 8
UR https://archimer.ifremer.fr/doc/00454/56534/58236.pdf
   https://archimer.ifremer.fr/doc/00454/56534/58237.xlsx
LA English
DT Article
DE ;biopreservation;Listeria monocytogenes;high-throughput screening assays;bioluminescence;competition;mixed culture;bioprotection;antibacterial activities
AB This article describes a method for high-throughput competition assays using a bioluminescent strain of L. monocytogenes. This method is based on the use of the luminescent indicator strain L. monocytogenes EGDelux. The luminescence of this strain is correlated to growth, which make it suitable to monitor the growth of L. monocytogenes in mixed cultures. To this aim, luminescence kinetics were converted into a single numerical value, called the Luminescence Disturbance Indicator (LDI), which takes into account growth inhibition phenomena resulting in latency increase, decrease in the luminescence rate, or reduction of the maximum luminescence. The LDI allows to automatically and simultaneously handle multiple competition assays which are required for high-throughput screening (HTS) approaches. The method was applied to screen a collection of 1810 strains isolated from raw cow’s milk in order to identify non-acidifying strains with anti-L. monocytogenes bioprotection properties. This method was also successfully used to identify anti-L. monocytogenes candidates within a collection of Lactococcus piscium, a species where antagonism was previously described as non-diffusible and requiring cell-to-cell contact. In conclusion, bioluminescent L. monocytogenes can be used in HTS to identify strains with anti-L. monocytogenes bioprotection properties, irrespectively of the inhibition mechanism. 
PY 2018
PD AUG
SO Frontiers In Microbiology
SN 1664-302X
PU Frontiers Media Sa
VL 9
IS 1883
UT 000441902900001
DI 10.3389/fmicb.2018.01883
ID 56534
ER

EF