Exploring the Impact of the Biofloc Rearing System and an Oral WSSV Challenge on the Intestinal Bacteriome of Litopenaeus vannamei

Type Article
Date 2018-09
Language English
Author(s) Pilotto Mariana R.1, Goncalves Andre N. A.1, Vieira Felipe N.2, Seifert Walter Q.2, Bachere Evelyne3, Rosa Rafael D.1, Perazzolo Luciane M.1
Affiliation(s) 1 : Univ Fed Santa Catarina, Dept Cell Biol Embryol & Genet, Lab Immunol Appl Aquaculture, BR-88040900 Florianopolis, SC, Brazil.
2 : Univ Fed Santa Catarina, Lab Marine Shrimp, BR-88040900 Florianopolis, SC, Brazil.
3 : Univ Montpellier, CNRS, IHPE Interact Hosts Pathogens Environm, UPVD,Ifremer,UMR 5244, F-34095 Montpellier, France.
Source Microorganisms (2076-2607) (Mdpi), 2018-09 , Vol. 6 , N. 3 , P. 83 (16p.)
DOI 10.3390/microorganisms6030083
WOS© Times Cited 21
Keyword(s) biofloc technology (BFT), penaeid shrimp, gut microbiota, White spot syndrome virus, 16S rRNA gene sequencing
Abstract

We provide a global overview of the intestinal bacteriome of Litopenaeus vannamei in two rearing systems and after an oral challenge by the White spot syndrome virus (WSSV). By using a high-throughput 16S rRNA gene sequencing technology, we identified and compared the composition and abundance of bacterial communities from the midgut of shrimp reared in the super-intensive biofloc technology (BFT) and clear seawater system (CWS). The predominant bacterial group belonged to the phylum Proteobacteria, followed by the phyla Bacteroidetes, Actinobacteria, and Firmicutes. Within Proteobacteria, the family Vibrionaceae, which includes opportunistic shrimp pathogens, was more abundant in CWS than in BFT-reared shrimp. Whereas the families Rhodobacteraceae and Enterobacteriaceae accounted for almost 20% of the bacterial communities of shrimp cultured in BFT, they corresponded to less than 3% in CWS-reared animals. Interestingly, the WSSV challenge dramatically changed the bacterial communities in terms of composition and abundance in comparison to its related unchallenged group. Proteobacteria remained the dominant phylum. Vibrionaceae was the most affected in BFT-reared shrimp (from 11.35 to 20.80%). By contrast, in CWS-reared animals the abundance of this family decreased from 68.23 to 23.38%. Our results provide new evidence on the influence of both abiotic and biotic factors on the gut bacteriome of aquatic species of commercial interest.

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Pilotto Mariana R., Goncalves Andre N. A., Vieira Felipe N., Seifert Walter Q., Bachere Evelyne, Rosa Rafael D., Perazzolo Luciane M. (2018). Exploring the Impact of the Biofloc Rearing System and an Oral WSSV Challenge on the Intestinal Bacteriome of Litopenaeus vannamei. Microorganisms, 6(3), 83 (16p.). Publisher's official version : https://doi.org/10.3390/microorganisms6030083 , Open Access version : https://archimer.ifremer.fr/doc/00454/56546/