Exploring the chemodiversity of tropical microalgae for the discovery of natural antifouling compounds

Type Article
Date 2019-02
Language English
Author(s) Réveillon DamienORCID1, 2, Tunin-Ley Alina3, Grondin Isabelle4, Othmani Ahlem1, Zubia Mayalen5, Bunet Robert6, Turquet Jean3, Culioli Gérald1, Briand Jean-François1
Affiliation(s) 1 : MAPIEM EA 4323, Université de Toulon, Toulon, France
2 : Laboratoire Phycotoxines, Ifremer, Nantes, France
3 : Hydrô Réunion, CBEM c/o CYROI, Sainte Clotilde, La Réunion, France
4 : Laboratoire de Chimie des Substances Naturelles et des Sciences des Aliments (LCSNSA), Université de La Réunion, Saint-Denis, France
5 : Université de Polynésie Française, UMR-EIO, LabEx-CORAIL, BP 6570, 98702 Faa’a, Tahiti, French Polynesia
6 : Institut Océanographique Paul Ricard, Ile des Embiez, Six-Fours-les-Plages, France
Source Journal Of Applied Phycology (0921-8971) (Springer Nature America, Inc), 2019-02 , Vol. 31 , N. 1 , P. 319-333
DOI 10.1007/s10811-018-1594-z
WOS© Times Cited 5
Keyword(s) Microalgae, Antifouling, Bioassay, Chemodiversity, Bioprospecting, Metabolomics
Abstract

Marine microalgae and cyanobacteria have largely been studied for their biotechnological potential and proved their ability to produce a wide array of bioactive molecules. We investigated the antifouling potential of unexplored benthic tropical microalgae using anti-adhesion and toxicity bioassays against two major micro- and ma crobiofoulers, namely bacteria and barnacles. Fifty strains belonging to six phyla [Cyanobacteria, Miozoa (Dinoflagellata), Bacillariophyta, Cryptophyta, Rhodophyta and Haptophyta] were isolated from southwestern Islands of the Indian Ocean. They were chosen in order to represent as much as possible the huge biodiversity of such a rich tropical ecosystem. The associated chemodiversity was highlighted by both NMR- and LC-MS-based metabolomics. The screening of 84 algal fractions revealed that the anti-adhesion activity was concentrated in methanolic ones (i.e. 93% of all active fractions). Our results confirmed that microalgae constitute a promising source of natural antimicrofoulants as 17 out of the 30 active fractions showed high or very high capacity to inhibit the adhesion of three biofilm-forming marine bacteria. Dinoflagellate-derived fractions were the most active, both in terms of number and intensity. However, dinoflagellates were also more toxic and may not be suitable as a source of environmentally friendly antifouling compounds, in contrast to diatoms, e.g. Navicula mollis. The latter and two dinoflagellates of the genus Amphidinium also had interesting anti-settlement activities while being moderately toxic to barnacle larvae. Our approach, combining the bioprospecting of a large number of tropical microalgae for their anti-settlement potential and metabolomics analyses, constituted a first step towards the discovery of alternative ecofriendly antifoulants.

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Réveillon Damien, Tunin-Ley Alina, Grondin Isabelle, Othmani Ahlem, Zubia Mayalen, Bunet Robert, Turquet Jean, Culioli Gérald, Briand Jean-François (2019). Exploring the chemodiversity of tropical microalgae for the discovery of natural antifouling compounds. Journal Of Applied Phycology, 31(1), 319-333. Publisher's official version : https://doi.org/10.1007/s10811-018-1594-z , Open Access version : https://archimer.ifremer.fr/doc/00454/56608/