FN Archimer Export Format
PT J
TI Molecular analyses of protists in long-term observation programmes—current status and future perspectives
BT 
AF STERN, Rowena
   KRABERG, Alexandra
   BRESNAN, Eileen
   KOOISTRA, Wiebe H. C. F.
   LOVEJOY, Connie
   MONTRESOR, Marina
   MORAN, Xose Anxelu G.
   NOT, Fabrice
   SALAS, Rafael
   SIANO, Raffaele
   VAULOT, Daniel
   AMARAL-ZETTLER, Linda
   ZINGONE, Adriana
   METFIES, Katja
AS 1:1;2:2;3:3;4:4;5:5;6:4;7:6;8:7,8;9:9;10:10;11:7,8;12:11;13:4;14:12,13;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:PDG-ODE-DYNECO-PELAGOS;11:;12:;13:;14:;
C1 Marine Biol Assoc UK, Citadel Hill, Plymouth PL1 2PB, Devon, England.
   Helmholtz Ctr Polar & Marine Res, Alfred Wegener Inst, Biol Anstalt Helgoland, Kurpromenade 201, D-27498 Helgoland, Germany.
   Marine Scotland Marine Lab, 375 Victoria Rd, Aberdeen AB11 9DB, Scotland.
   Stn Zool Anton Dohrn, Dept Integrat Marine Ecol, I-80121 Naples, Italy.
   Univ Laval, Quebec Ocean, Dept Biol, IBIS, Quebec City, PQ G1V 0A6, Canada.
   King Abdullah Univ Sci & Technol, Biol & Environm Sci & Engn Div, Red Sea Res Ctr, Thuwal 239556900, Saudi Arabia.
   UPMC Univ Paris 06, Sorbonne univ, CNRS, UMR 7144, Paris, France.
   Stn Biol, Pl Georges Teissier, F-29680 Roscoff, France.
   Marine Inst, Marine Environm & Food Safety Serv, Rinville H9I R673, Oranmore, Ireland.
   IFREMER, Ctr Brest, Dept DYNECO, BP70, F-29280 Plouzane, France.
   NIOZ, Dept Microbiol & Biogeochem, POB 59, NL-1790 AB Den Burg, Texel, Netherlands.
   Helmholtz Ctr Polar & Marine Res, Alfred Wegener Inst, D-27515 Bremerhaven, Germany.
   Carl von Ossietzky Univ Oldenburg, Helmholtz Inst Funct Marine Biodivers, D-26129 Oldenburg, Germany.
C2 MBA, UK
   INST A WEGENER, GERMANY
   MARINE SCOTLAND SCI (NRL), UK
   STN ZOOL ANTON DOHRN, ITALY
   UNIV LAVAL, CANADA
   UNIV KING ABDULLAH, SAUDI ARABIA
   UNIV PARIS 06, FRANCE
   CNRS, FRANCE
   MARINE INST, IRELAND
   IFREMER, FRANCE
   INST SEA RESEARCH (NIOZ), NETHERLANDS
   INST A WEGENER, GERMANY
   UNIV OLDENBURG, GERMANY
SI BREST
SE PDG-ODE-DYNECO-PELAGOS
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-europe
   copubli-univ-france
   copubli-int-hors-europe
IF 2.209
TC 37
UR https://archimer.ifremer.fr/doc/00455/56708/58470.pdf
   https://archimer.ifremer.fr/doc/00455/56708/58471.pdf
   https://archimer.ifremer.fr/doc/00455/56708/58472.xlsx
   https://archimer.ifremer.fr/doc/00455/56708/58473.xlsx
LA English
DT Article
DE ;protists;Long-term Ecological Research Station;molecular;time-series;questionnaire;literature survey
AB Protists (microbial eukaryotes) are diverse, major components of marine ecosystems, and are fundamental to ecosystem services. In the last 10 years, molecular studies have highlighted substantial novel diversity in marine systems including sequences with no taxonomic context. At the same time, many known protists remain without a DNA identity. Since the majority of pelagic protists are too small to identify by light microscopy, most are neither comprehensively or regularly taken into account, particularly in Long-term Ecological Research Sites. This potentially undermines the quality of research and the accuracy of predictions about biological species shifts in a changing environment. The ICES Working Group for Phytoplankton and Microbial Ecology conducted a questionnaire survey in 2013–2014 on methods and identification of protists using molecular methods plus a literature review of protist molecular diversity studies. The results revealed an increased use of high-throughput sequencing methods and a recognition that sequence data enhance the overall datasets on protist species composition. However, we found only a few long-term molecular studies and noticed a lack of integration between microscopic and molecular methods. Here, we discuss and put forward recommendations to improve and make molecular methods more accessible to Long-term Ecological Research Site investigators. 
PY 2018
PD SEP
SO Journal Of Plankton Research
SN 0142-7873
PU Oxford Univ Press
VL 40
IS 5
UT 000446087300002
BP 519
EP 536
DI 10.1093/plankt/fby035
ID 56708
ER

EF