FN Archimer Export Format
PT J
TI Protists Within Corals: The Hidden Diversity
BT 
AF CLERISSI, Camille
   BRUNET, Sebastien
   VIDAL-DUPIOL, Jeremie
   ADJEROUD, Mehdi
   LEPAGE, Pierre
   GUILLOU, Laure
   ESCOUBAS, Jean Michel
   TOULZA, Eve
AS 1:1,4;2:2,3;3:4;4:5,6;5:2,3;6:7;7:1;8:1;
FF 1:PDG-RBE-IHPE;2:;3:PDG-RBE-IHPE;4:;5:;6:;7:;8:;
C1 Univ Montpellier, Univ Perpignan Via Domitia, CNRS, IHPE UMR 5244,IFREMER, Perpignan, France.
   McGill Univ, Montreal, PQ, Canada.
   Genome Quebec Innovat Ctr, Montreal, PQ, Canada.
   IFREMER, IHPE UMR 5244, Univ. Perpignan Via Domitia, CNRS, Univ. Montpellier, Montpellier, France
   Univ Perpignan, Inst Rech & Dev, UMR ENTROPIE 9220, Perpignan, France.
   Univ Perpignan, Lab Excellence CORAIL, Perpignan, France.
   Univ Pierre & Marie Curie Paris 6, Stn Biol Roscoff, Sorbonne Univ, CNRS,UMR 7144, Roscoff, France.
C2 UNIV PERPIGNAN, FRANCE
   UNIV MCGILL, CANADA
   GENOME QUEBEC INNOVAT CTR, CANADA
   IFREMER, FRANCE
   IRD, FRANCE
   CNRS, FRANCE
   CNRS, FRANCE
SI MONTPELLIER
SE PDG-RBE-IHPE
UM IHPE
IN WOS Ifremer jusqu'en 2018
   DOAJ
   copubli-france
   copubli-p187
   copubli-univ-france
   copubli-int-hors-europe
IF 4.259
TC 21
UR https://archimer.ifremer.fr/doc/00457/56823/58661.pdf
   https://archimer.ifremer.fr/doc/00457/56823/58662.tiff
   https://archimer.ifremer.fr/doc/00457/56823/58663.tiff
   https://archimer.ifremer.fr/doc/00457/56823/58664.tiff
   https://archimer.ifremer.fr/doc/00457/56823/58665.tiff
   https://archimer.ifremer.fr/doc/00457/56823/58666.xlsx
   https://archimer.ifremer.fr/doc/00457/56823/58667.xlsx
   https://archimer.ifremer.fr/doc/00457/56823/58668.xlsx
   https://archimer.ifremer.fr/doc/00457/56823/58669.xlsx
   https://archimer.ifremer.fr/doc/00457/56823/58670.xlsx
LA English
DT Article
DE ;holobiont;protists;symbiosis;metabarcoding;blocking primer;Scleractinia;Pocillopora damicornis
AB Previous observations suggested that microbial communities contribute to coral health and the ecological resilience of coral reefs. However, most studies of coral microbiology focused on prokaryotes and the endosymbiotic algae Symbiodinium. In contrast, knowledge concerning diversity of other protists is still lacking, possibly due to methodological constraints. As most eukaryotic DNA in coral samples was derived from hosts, protist diversity was missed in metagenome analyses. To tackle this issue, we designed blocking primers for Scleractinia sequences amplified with two primer sets that targeted variable loops of the 18S rRNA gene (18SV1V2 and 18SV4). These blocking primers were used on environmental colonies of Pocillopora damicornis sensu lato from two regions with contrasting thermal regimes (Djibouti and New Caledonia). In addition to Symbiodinium clades A/C/D, Licnophora and unidentified coccidia genera were found in many samples. In particular, coccidian sequences formed a robust monophyletic clade with other protists identified in Agaricia, Favia, Montastraea, Mycetophyllia, Porites, and Siderastrea coral colonies. Moreover, Licnophora and coccidians had different distributions between the two geographic regions. A similar pattern was observed between Symbiodinium clades C and A/D. Although we were unable to identify factors responsible for this pattern, nor were we able to confirm that these taxa were closely associated with corals, we believe that these primer sets and the associated blocking primers offer new possibilities to describe the hidden diversity of protists within different coral species. 
PY 2018
PD AUG
SO Frontiers In Microbiology
SN 1664-302X
PU Frontiers Media Sa
VL 9
UT 000443237100001
DI 10.3389/fmicb.2018.02043
ID 56823
ER

EF