FN Archimer Export Format
PT J
TI Inorganic carbon and nitrogen assimilation in cellular compartments of a benthic kleptoplastic foraminifer
BT 
AF LEKIEFFRE, Charlotte
   JAUFFRAIS, Thierry
   GESLIN, Emmanuelle
   JESUS, Bruno
   BERNHARD, Joan M.
   GIOVANI, Maria-Evangelia
   MEIBOM, Anders
AS 1:1,2;2:2;3:2;4:3,4;5:5;6:1;7:1,6;
FF 1:;2:;3:;4:;5:;6:;7:;
C1 Ecole Polytech Fed Lausanne, Sch Architecture Civil & Environm Engn ENAC, Lab Biol Geochem, CH-1015 Lausanne, Switzerland.
   Univ Angers, UMR CNRS LPG BIAF 6112, 2 Blvd Lavoisier, F-49045 Angers 1, France.
   Univ Nantes, Lab Mer Mol Sante, EA2160, Nantes, France.
   Campo Grande Univ Lisboa, BioISI Biosyst Integrat Sci Inst, Fac Sci, Lisbon, Portugal.
   Woods Hole Oceanog Inst, Dept Geol & Geophys, Woods Hole, MA 02543 USA.
   Univ Lausanne, Inst Earth Sci, Ctr Adv Surface Anal, CH-1015 Lausanne, Switzerland.
C2 ECOLE POLYTECH FED LAUSANNE, SWITZERLAND
   UNIV ANGERS, FRANCE
   UNIV NANTES, FRANCE
   UNIV LISBOA, PORTUGAL
   WHOI, USA
   UNIV LAUSANNE, SWITZERLAND
IN DOAJ
IF 4.011
TC 28
UR https://archimer.ifremer.fr/doc/00457/56887/58761.pdf
   https://archimer.ifremer.fr/doc/00457/56887/58762.pdf
LA English
DT Article
AB Haynesina germanica, an ubiquitous benthic foraminifer in intertidal mudflats, has the remarkable ability to isolate, sequester, and use chloroplasts from microalgae. The photosynthetic functionality of these kleptoplasts has been demonstrated by measuring photosystem II quantum efficiency and O-2 production rates, but the precise role of the kleptoplasts in foraminiferal metabolism is poorly understood. Thus, the mechanism and dynamics of C and N assimilation and translocation from the kleptoplasts to the foraminiferal host requires study. The objective of this study was to investigate, using correlated TEM and NanoSIMS imaging, the assimilation of inorganic C and N (here ammonium, NH4+) in individuals of a kleptoplastic benthic foraminiferal species. H. germanica specimens were incubated for 20 h in artificial seawater enriched with (HCO3-)-C-13 and (NH4+)-N-15 during a light/dark cycle. All specimens (n = 12) incorporated C-13 into their endoplasm stored primarily in the form of lipid droplets. A control incubation in darkness resulted in no C-13-uptake, strongly suggesting that photosynthesis is the process dominating inorganic C assimilation. Ammonium assimilation was observed both with and without light, with diffuse N-15-enrichment throughout the cytoplasm and distinct N-15-hotspots in fibrillar vesicles, electron-opaque bodies, tubulin paracrystals, bacterial associates, and, rarely and at moderate levels, in kleptoplasts. The latter observation might indicate that the kleptoplasts are involved in N assimilation. However, the higher N assimilation observed in the foraminiferal endoplasm incubated without light suggests that another cytoplasmic pathway is dominant, at least in darkness. This study clearly shows the advantage provided by the kleptoplasts as an additional source of carbon and provides observations of ammonium uptake by the foraminiferal cell. 
PY 2018
PD JUN
SO Scientific Reports
SN 2045-2322
PU Nature Publishing Group
VL 8
IS 10140
UT 000437249500004
DI 10.1038/s41598-018-28455-1
ID 56887
ER

EF