FN Archimer Export Format
PT J
TI Kleptoplastidic benthic foraminifera from aphotic habitats: Insights into assimilation of inorganic C, N, and S studied with sub-cellular resolution
BT 
AF JAUFFRAIS, Thierry
   LEKIEFFRE, Charlotte
   SCHWEIZER, Magali
   GESLIN, Emmanuelle
   METZGER, Edouard
   BERNHARD, Joan M.
   JESUS, Bruno
   FILIPSSON, Helena L.
   MAIRE, Olivier
   MEIBOM, Anders
AS 1:1,2;2:1,3;3:1;4:1;5:1;6:4;7:5,6;8:7;9:8,9;10:3,10;
FF 1:PDG-RBE-LEADNC;2:;3:;4:;5:;6:;7:;8:;9:;10:;
C1 Univ Angers, UMR CNRS 6112 LPG BIAF, 2 Blvd Lavoisier, F-49045 Angers 1, France.
   IFREMER, RBE LEAD, 101 Promenade Roger Laroque, Noumea 98897, New Caledonia.
   Ecole Polytech Fed Lausanne, Sch Architecture Civil & Environm Engn ENAC, Lab Biol Geochem, CH-1015 Lausanne, Switzerland.
   Woods Hole Oceanog Inst, Dept Geol & Geophys, Woods Hole, MA 02543 USA.
   Univ Nantes, Lab Mer Mol Sante, EA2160, Nantes, France.
   Campo Grande Univ Lisboa, Fac Sci, BioISI Biosyst & Integrat Sci Inst, Lisbon, Portugal.
   Lund Univ, Dept Geol, Solvegatan 12, S-22362 Lund, Sweden.
   Univ Bordeaux, EPOC, UMR 5805, F-33400 Talence, France.
   CNRS, EPOC, UMR 5805, F-33400 Talence, France.
   Univ Lausanne, Inst Earth Sci, Ctr Adv Surface Anal, CH-1015 Lausanne, Switzerland.
C2 UNIV ANGERS, FRANCE
   IFREMER, FRANCE
   EPFL, SWITZERLAND
   WHOI, USA
   UNIV NANTES, FRANCE
   UNIV LISBOA, PORTUGAL
   UNIV LUND, SWEDEN
   UNIV BORDEAUX, FRANCE
   CNRS, FRANCE
   UNIV LAUSANNE, SWITZERLAND
SI NOUMEA
SE PDG-RBE-LEADNC
IN WOS Ifremer UPR
   copubli-france
   copubli-europe
   copubli-univ-france
   copubli-int-hors-europe
IF 4.933
TC 25
UR https://archimer.ifremer.fr/doc/00460/57188/59151.pdf
LA English
DT Article
AB The assimilation of inorganic compounds in foraminiferal metabolism compared to predation or organic matter assimilation is unknown. Here we investigate possible inorganic‐compound assimilation in Nonionellina labradorica, a common kleptoplastidic benthic foraminifer from Arctic and North Atlantic sublittoral regions. The objectives were to identify the source of the foraminiferal kleptoplasts, assess their photosynthetic functionality in light and darkness, and investigate inorganic nitrogen and sulfate assimilation. We used DNA barcoding of a ~830 bp fragment from the SSU rDNA to identify the kleptoplasts, and correlated transmission electron microscopy and nanometer‐scale secondary ion mass spectrometry (TEM‐NanoSIMS) isotopic imaging to study 13C‐bicarbonate, 15N‐ammonium, and 34S‐sulfate uptake. In addition, respiration rate measurements were determined to assess the response of N. labradorica to light. The DNA sequences established that over 80% of the kleptoplasts belonged to Thalassiosira (with 96‐99% identity), a cosmopolitan planktonic diatom. TEM‐NanoSIMS imaging revealed degraded cytoplasm and an absence of 13C assimilation in foraminifera exposed to light. Oxygen measurements showed higher respiration rates under light than dark conditions, and no O2 production was detected. These results indicate that the photosynthetic pathways in N. labradorica are not functional. Furthermore, N. labradorica assimilated both 15N‐ammonium and 34S‐sulfate into its cytoplasm, which suggests that foraminifera might have several ammonium or sulfate assimilation pathways, involving either the kleptoplasts or bona fide foraminiferal pathway(s) not yet identified. 
PY 2019
PD JAN
SO Environmental Microbiology
SN 1462-2912
PU Wiley
VL 21
IS 1
UT 000456278900009
BP 125
EP 141
DI 10.1111/1462-2920.14433
ID 57188
ER

EF