Immune-suppression by OsHV-1 viral infection causes fatal bacteraemia in Pacific oysters

Type Article
Date 2018-10
Language English
Author(s) De Lorgeril Julien1, Lucasson Aude1, Petton Bruno2, Toulza Eve1, Montagnani Caroline1, Clerissi Camille1, Vidal-Dupiol Jeremie1, Chaparro Cristian1, Galinier Richard1, Escoubas Jean Michel1, Haffner Philippe1, Degremont LionelORCID3, Charriere Guillaume1, Lafont Maxime1, Delort Abigail1, Vergnes Agnes1, Chiarello Marlene4, Faury Nicole3, Rubio Tristan1, Leroy Marc1, Perignon Adeline5, Regler Denis5, Morga Benjamin3, Alunno-Bruscia MarianneORCID2, Boudry PierreORCID6, Le Roux Frederique7, Destoumieux-Garzon DelphineORCID1, Gueguen YannickORCID1, Mitta Guillaume1
Affiliation(s) 1 : Univ Perpignan, Univ Montpellier, IFREMER, IHPE,CNRS, Via Domitia,Pl E Bataillon, F-34095 Montpellier, France.
2 : UBO, CNRS, IRD, LEMAR UMR 6539,Ifremer, 11 Presquile Vivier, F-29840 Argenton En Landunvez, France.
3 : IFREMER, Lab Genet & Pathol Mollusques Marins, Ave Mus de Loup, F-17930 La Tremblade, France.
4 : Univ Montpellier, CNRS, IFREMER, Marine Biodivers Exploitat & Conservat MARBEC,IR, Pl E Bataillon, F-34095 Montpellier, France.
5 : CRCM, Com Conchyliculture Mediterranee, F-34140 Meze, France.
6 : UBO, IRD, IFREMER, LEMAR UMR6539,CNRS, ZI Pointe Diable,CS 10070, F-29280 Plouzane, France.
7 : UPMC Paris 06, Sorbonne Univ, CNRS, UMR 8227,LBI2M,Ifremer,Stn Biol Roscoff, CS 90074, F-29680 Roscoff, France.
Source Nature Communications (2041-1723) (Nature Publishing Group), 2018-10 , Vol. 9 , N. 4215 , P. 14p.
DOI 10.1038/s41467-018-06659-3
WOS© Times Cited 6
Abstract

Infectious diseases are mostly explored using reductionist approaches despite repeated evidence showing them to be strongly in fluenced by numerous interacting host and environmental factors. Many diseases with a complex aetiology therefore remain misunderstood. By developing a holistic approach to tackle the complexity of interactions, we decipher the complex intra-host interactions underlying Pacific oyster mortality syndrome affecting juveniles of Crassostrea gigas, the main oyster species exploited worldwide. Using experimental infections reproducing the natural route of infection and combining thorough molecular analyses of oyster families with contrasted susceptibilities, we demonstrate that the disease is caused by multiple infection with an initial and necessary step of infection of oyster haemocytes by the Ostreid herpesvirus OsHV-1 μVar. Viral replication leads to the host entering an immune-compromised state, evolving towards subsequent bacteraemia by opportunistic bacteria. We propose the application of our integrative approach to decipher other multifactorial diseases that affect non-model species worldwide.

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Publisher's official version 14 4 MB Open access
Supplementary Information 26 3 MB Open access
Peer Review File 16 513 KB Open access
Description of Additional Supplementary Files 1 167 KB Open access
Supplementary Data 1 1 MB Open access
Supplementary Data 2 18 KB Open access
Supplementary Data 3 33 KB Open access
Supplementary Data 4 31 KB Open access
Supplementary Data 5 505 KB Open access
Supplementary Data 6 18 KB Open access
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De Lorgeril Julien, Lucasson Aude, Petton Bruno, Toulza Eve, Montagnani Caroline, Clerissi Camille, Vidal-Dupiol Jeremie, Chaparro Cristian, Galinier Richard, Escoubas Jean Michel, Haffner Philippe, Degremont Lionel, Charriere Guillaume, Lafont Maxime, Delort Abigail, Vergnes Agnes, Chiarello Marlene, Faury Nicole, Rubio Tristan, Leroy Marc, Perignon Adeline, Regler Denis, Morga Benjamin, Alunno-Bruscia Marianne, Boudry Pierre, Le Roux Frederique, Destoumieux-Garzon Delphine, Gueguen Yannick, Mitta Guillaume (2018). Immune-suppression by OsHV-1 viral infection causes fatal bacteraemia in Pacific oysters. Nature Communications, 9(4215), 14p. Publisher's official version : https://doi.org/10.1038/s41467-018-06659-3 , Open Access version : https://archimer.ifremer.fr/doc/00461/57234/