FN Archimer Export Format
PT J
TI Occurrence of Bacterial Pathogens and Human Noroviruses in Shellfish-Harvesting Areas and Their Catchments in France
BT 
AF RINCE, Alain
   BALIERE, Charlotte
   HERVIO-HEATH, Dominique
   COZIEN, Joelle
   LOZACH, Solen
   PARNAUDEAU, Sylvain
   LE GUYADER, Francoise S.
   LE HELLO, Simon
   GIARD, Jean-Christophe
   SAUVAGEOT, Nicolas
   BENACHOUR, Abdellah
   STRUBBIA, Sofia
   GOURMELON, Michele
AS 1:1;2:2;3:2;4:2;5:2;6:2;7:2;8:3;9:1;10:1;11:1;12:3;13:2;
FF 1:;2:PDG-RBE-SGMM-LSEM;3:PDG-RBE-SGMM-LSEM;4:;5:PDG-RBE-SGMM-LSEM;6:PDG-RBE-SGMM-LSEM;7:;8:;9:;10:;11:;12:PDG-RBE-SGMM-LSEM;13:PDG-RBE-SGMM-LSEM;
C1 Normandie Univ, U2RM, UNICAEN, Caen, France.
   Inst Francais Rech Exploitat Mer, RBE SG2M LSEM, Brest, France.
   Inst Pasteur, Unite Batteries Pathogens Enter, Paris, France.
C2 UNIV NORMANDIE, FRANCE
   IFREMER, FRANCE
   INST PASTEUR, FRANCE
SI BREST
   NANTES
SE PDG-RBE-SGMM-LSEM
IN WOS Ifremer jusqu'en 2018
   DOAJ
   copubli-france
   copubli-univ-france
IF 4.259
TC 30
UR https://archimer.ifremer.fr/doc/00461/57243/59243.pdf
   https://archimer.ifremer.fr/doc/00461/57243/59244.pdf
   https://archimer.ifremer.fr/doc/00461/57243/59245.pdf
   https://archimer.ifremer.fr/doc/00461/57243/59246.pdf
   https://archimer.ifremer.fr/doc/00461/57243/59247.pdf
LA English
DT Article
DE ;Campylobacter;Salmonella;Vibrio;HuNoVs;fecal bacterial indicators;shellfish;water
AB During a 2-year study, the presence of human pathogenic bacteria and noroviruses was investigated in shellfish, seawater and/or surface sediments collected from three French coastal shellfish-harvesting areas as well as in freshwaters from the corresponding upstream catchments. Bacteria isolated from these samples were further analyzed. Escherichia coli isolates classified into the phylogenetic groups B2, or D and enterococci from Enterococcus faecalis and E. faecium species were tested for the presence of virulence genes and for antimicrobial susceptibility. Salmonella members were serotyped and the most abundant serovars (Typhimurium and its monophasic variants and Mbandaka) were genetically characterized by high discriminative subtyping methods. Campylobacter and Vibrio were identified at the species level, and haemolysin-producing Vibrio parahaemolyticus were searched by tdh- and trh- gene detection. Main results showed a low prevalence of Salmonella in shellfish samples where only members of S. Mbandaka were found. Campylobacter were more frequently isolated than Salmonella and a different distribution of Campylobacter species was observed in shellfish compared to rivers, strongly suggesting possible additional inputs of bacteria. Statistical associations between enteric bacteria, human noroviruses (HuNoVs) and concentration of fecal indicator bacteria revealed that the presence of Salmonella was correlated with that of Campylobacter jejuni and/or C. coli as well as to E. coli concentration. A positive correlation was also found between the presence of C. lari and the detection of HuNoVs. This study highlights the importance of simultaneous detection and characterization of enteric and marine pathogenic bacteria and human noroviruses not only in shellfish but also in catchment waters for a hazard assessment associated with microbial contamination of shellfish. 
PY 2018
PD OCT
SO Frontiers In Microbiology
SN 1664-302X
PU Frontiers Media Sa
VL 9
UT 000447012100001
DI 10.3389/fmicb.2018.02443
ID 57243
ER

EF