Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae

Type Article
Date 2018-10
Language English
Author(s) Foulon Valentin1, Artigaud Sebastien1, Buscaglia Manon1, Bernay Benoit2, Fabioux Caroline1, Petton Bruno3, Elies Philippe4, Boukerma KadaORCID5, Hellio Claire1, Guerard Fabienne1, Boudry PierreORCID3
Affiliation(s) 1 : Inst Univ Europeen Mer, UMR CNRS UBO IRD Ifremer 6539, Lab Sci Environm Marin LEMAR, Technopole Brest Iroise,Rue Dumont dUrville, F-29280 Plouzane, France.
2 : Univ Caen Basse Normandie, Plateforme Proteogen SF ICORE, F-14032 Caen, France.
3 : IFREMER, UMR CNRS UBO IRD Ifremer 6539, Ctr Bretagne, Lab Sci Environm Marin, F-29280 Plouzane, France.
4 : Univ Bretagne Occidentale, Plateforme Imagerie & Mesures Microscopie, F-29200 Brest, France.
5 : IFREMER, Ctr Bretagne, LDCM, F-29280 Plouzane, France.
Source Scientific Reports (2045-2322) (Nature Publishing Group), 2018-10 , Vol. 8 , N. 1 , P. 15298 (14p.)
DOI 10.1038/s41598-018-33720-4
WOS© Times Cited 2
Abstract

Bioadhesion of marine organisms has been intensively studied over the last decade because of their ability to attach in various wet environmental conditions and the potential this offers for biotechnology applications. Many marine mollusc species are characterized by a two-phase life history: pelagic larvae settle prior to metamorphosis to a benthic stage. The oyster Crassostrea gigas has been extensively studied for its economic and ecological importance. However, the bioadhesive produced by ready to settle larvae of this species has been little studied. The pediveliger stage of oysters is characterized by the genesis of a specific organ essential for adhesion, the foot. Our scanning electron microscopy and histology analysis revealed that in C. gigas the adhesive is produced by several foot glands. This adhesive is composed of numerous fibres of differing structure, suggesting differences in chemical composition and function. Fourier transformed infrared spectroscopy indicated a mainly proteinaceous composition. Proteomic analysis of footprints was able to identify 42 proteins, among which, one uncharacterized protein was selected on the basis of its pediveliger transcriptome specificity and then located by mRNA in situ hybridization, revealing its potential role during substrate exploration before oyster larva settlement.

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Foulon Valentin, Artigaud Sebastien, Buscaglia Manon, Bernay Benoit, Fabioux Caroline, Petton Bruno, Elies Philippe, Boukerma Kada, Hellio Claire, Guerard Fabienne, Boudry Pierre (2018). Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae. Scientific Reports, 8(1), 15298 (14p.). Publisher's official version : https://doi.org/10.1038/s41598-018-33720-4 , Open Access version : https://archimer.ifremer.fr/doc/00462/57379/