FN Archimer Export Format
PT J
TI NanoSIMS single cell analyses reveal the contrasting nitrogen sources for small phytoplankton
BT 
AF Berthelot, Hugo
   Duhamel, Solange
   L’Helguen, Stéphane
   Maguer, Jean-Francois
   Wang, Seaver
   Cetinić, Ivona
   Cassar, Nicolas
AS 1:1;2:2;3:1;4:1;5:3;6:5;7:1,3;
FF 1:;2:;3:;4:;5:;6:;7:;
C1 Laboratoire des Sciences de l’Environnement Marin (LEMAR), UMR 6539 UBO/CNRS/IRD/IFREMER, Institut Universitaire Européen de la Mer (IUEM), Brest, France
   Division of Biology and Paleo Environment, Lamont-Doherty Earth Observatory, PO Box 1000, 61 Route 9W, Palisades, NY, 10964, USA
   Division of Earth and Ocean Sciences, Nicholas School of the Environment, Duke University, Durham, NC, 27708, USA
   NASA Goddard Space Flight Center, Ocean Ecology Laboratory, Code 616, Greenbelt, MD, USA
   GESTAR/Universities Space Research Association, Columbia, MD, USA
C2 UBO, FRANCE
   UNIV COLUMBIA, USA
   UNIV DUKE, USA
   NASA, USA
   USRA, USA
UM LEMAR
IN WOS Cotutelle UMR
   copubli-int-hors-europe
IF 9.18
TC 56
UR https://archimer.ifremer.fr/doc/00462/57383/59462.pdf
   https://archimer.ifremer.fr/doc/00462/57383/59463.pdf
   https://archimer.ifremer.fr/doc/00462/57383/64185.pdf
   https://archimer.ifremer.fr/doc/00462/57383/77969.pdf
LA English
DT Article
AB Nitrogen (N) is a limiting nutrient in vast regions of the world’s oceans, yet the sources of N available to various phytoplankton groups remain poorly understood. In this study, we investigated inorganic carbon (C) fixation rates and nitrate (NO3−), ammonium (NH4+) and urea uptake rates at the single cell level in photosynthetic pico-eukaryotes (PPE) and the cyanobacteria Prochlorococcus and Synechococcus. To that end, we used dual 15N and 13C-labeled incubation assays coupled to flow cytometry cell sorting and nanoSIMS analysis on samples collected in the North Pacific Subtropical Gyre (NPSG) and in the California Current System (CCS). Based on these analyses, we found that photosynthetic growth rates (based on C fixation) of PPE were higher in the CCS than in the NSPG, while the opposite was observed for Prochlorococcus. Reduced forms of N (NH4+ and urea) accounted for the majority of N acquisition for all the groups studied. NO3− represented a reduced fraction of total N uptake in all groups but was higher in PPE (17.4 ± 11.2% on average) than in Prochlorococcus and Synechococcus (4.5 ± 6.5 and 2.9 ± 2.1% on average, respectively). This may in part explain the contrasting biogeography of these picoplankton groups. Moreover, single cell analyses reveal that cell-to-cell heterogeneity within picoplankton groups was significantly greater for NO3− uptake than for C fixation and NH4+ uptake. We hypothesize that cellular heterogeneity in NO3− uptake within groups facilitates adaptation to the fluctuating availability of NO3− in the environment. 
PY 2019
PD MAR
SO Isme Journal
SN 1751-7362
PU Springer Nature America, Inc
VL 13
IS 3
UT 000459053600007
BP 651
EP 662
DI 10.1038/s41396-018-0285-8
ID 57383
ER

EF