FN Archimer Export Format
PT J
TI Differential Activities of DNA Polymerases in Processing Ribonucleotides during DNA Synthesis in Archaea
BT 
AF LEMOR, Melanie
   KONG, Ziqing
   HENRY, Etienne
   BRIZARD, Raphael
   LAURENT, Sebastien
   BOSSE, Audrey
   HENNEKE, Ghislaine
AS 1:3;2:2;3:4;4:1;5:1;6:1;7:1;
FF 1:;2:;3:;4:PDG-REM-EEP-LMEE;5:PDG-REM-EEP-LMEE;6:PDG-REM-EEP-LMEE;7:PDG-REM-EEP-LMEE;
C1 Univ Brest, CNRS, Lab Microbiol Environm Extremes, IFREMER, F-29280 Plouzane, France.
   Umea Univ, Dept Med Biochem & Biophys, Umea, Sweden.
   Univ Brest, CNRS, Lab Microbiol Environm Extremes, IFREMER, F-29280 Plouzane, France.
   Univ Brest, CNRS, Lab Microbiol Environm Extremes, IFREMER, F-29280 Plouzane, France.
C2 IFREMER, FRANCE
   UNIV UMEA, SWEDEN
   UBO, FRANCE
   CNRS, FRANCE
SI BREST
SE PDG-REM-EEP-LMEE
UM BEEP-LM2E
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-europe
   copubli-univ-france
IF 5.067
TC 11
UR https://archimer.ifremer.fr/doc/00464/57603/59796.pdf
   https://archimer.ifremer.fr/doc/00464/57603/59797.pdf
LA English
DT Article
DE ;Archaea;DNA replication and repair;DNA polymerase;Nucleotide pool;Translesion synthesis
AB Consistent with the fact that ribonucleotides (rNTPs) are in excess over deoxyribonucleotides (dNTPs) in vivo, recent findings indicate that replicative DNA polymerases (DNA Pols) are able to insert ribonucleotides (rNMPs) during DNA synthesis, raising crucial questions about the fidelity of DNA replication in both Bacteria and Eukarya. Here, we report that the level of rNTPs is 20-fold higher than that of dNTPs in Pyrococcus abyssi cells. Using dNTP and rNTP concentrations present in vivo, we recorded rNMP incorporation in a template-specific manner during in vitro synthesis, with the family-D DNA Pol (PolD) having the highest propensity compared with the family-B DNA Pol and the p41/p46 complex. We also showed that ribonucleotides accumulate at a relatively high frequency in the genome of wild-type Thermococcales cells, and this frequency significantly increases upon deletion of RNase HII, the major enzyme responsible for the removal of RNA from DNA. Because ribonucleotides remain in genomic DNA, we then analyzed the effects on polymerization activities by the three DNA Pols. Depending on the identity of the base and the sequence context, all three DNA Pols bypass rNMP-containing DNA templates with variable efficiency and nucleotide (mis)incorporation ability. Unexpectedly, we found that PolD correctly base-paired a single ribonucleotide opposite rNMP-containing DNA templates. An evolutionary scenario is discussed concerning rNMP incorporation into DNA and genome stability. 
PY 2018
PD DEC
SO Journal Of Molecular Biology
SN 0022-2836
PU Academic Press Ltd- Elsevier Science Ltd
VL 430
IS 24
UT 000454377100007
BP 4908
EP 4924
DI 10.1016/j.jmb.2018.10.004
ID 57603
ER

EF