Metabolomics based on UHPLC-QToF- and APGC-QToF-MS reveals metabolic pathways reprogramming in response to tidal cycles in the sub-littoral species Mimachlamys varia exposed to aerial emergence

Type Article
Date 2019-03
Language English
Author(s) Ory Pascaline1, Bonnet Antoine1, Mondeguer FlorenceORCID2, Breitwieser Marine1, Dubillot Emmanuel1, Graber Marianne1
Affiliation(s) 1 : Littoral Environnement et Sociétés (LIENSs), UMR 7266, CNRS-Université de La Rochelle, 2 rue Olympe de Gouges, F-17042 La Rochelle Cedex 01, France
2 : Ifremer, Laboratoire Phycotoxines, Rue de l'Ile d'Yeu, 44311 Nantes Cedex, France
Source Comparative Biochemistry And Physiology D-genomics & Proteomics (1744-117X) (Elsevier BV), 2019-03 , Vol. 29 , P. 74-85
DOI 10.1016/j.cbd.2018.11.002
Keyword(s) Anaerobic, APGC-QToF mass spectrometry, Hypoxia, Intertidal, LC-QToF mass spectrometry, Metabolomics, Mimachlamys varia, PLS-DA
Abstract

Mimachlamys varia is a sub-littoral bivalve encountered from Norway to the Mediterranean Sea, which lives mostly byssally attached to rocks. During the low tide period, M. varia individuals, located highest on the shore, may experience short time of aerial exposure and face a low availability of oxygen. Here we report a comparative metabolomic profiling of gill samples of M. varia obtained by both LC-QToF and APGC-QToF mass spectrometry, to analyze metabolic changes occurring during emersion in comparison with immersion. Scallops were grown in aquaria with a simulated intertidal environment mimicking short-duration air exposure that they might experience during extreme tides: alternating 2 h emersion and 10 h immersion. Our results show a switch from aerobic to anaerobic metabolism after only 2 h of emersion, with the resort to different pathways: glucose-lactate, glucose-succinate and aspartate-succinate pathways. Furthermore, carnitine-conjugated metabolites were found to accumulate during emersion, as well as urate. The level of tyrosine on the contrary was found to decrease. These findings indicate a complex metabolic reprogramming that occurs after a two hour emersion period and upon re-immersion. Furthermore, M. varia is used as sentinel species in pollution biomonitoring, through the assay of biomarkers to evaluate the effects of pollutants. Here we show that emersion induces a significant decrease of superoxide dismutase activity, an enzyme developed by bivalves to face oxidative stress and used as biomarker. These findings have to be taken into account to normalize sampling during campaigns of environmental monitoring, by taking in situ, as far as possible only immersed individuals.

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Ory Pascaline, Bonnet Antoine, Mondeguer Florence, Breitwieser Marine, Dubillot Emmanuel, Graber Marianne (2019). Metabolomics based on UHPLC-QToF- and APGC-QToF-MS reveals metabolic pathways reprogramming in response to tidal cycles in the sub-littoral species Mimachlamys varia exposed to aerial emergence. Comparative Biochemistry And Physiology D-genomics & Proteomics, 29, 74-85. Publisher's official version : https://doi.org/10.1016/j.cbd.2018.11.002 , Open Access version : https://archimer.ifremer.fr/doc/00466/57741/