FN Archimer Export Format
PT J
TI Metabolomics based on UHPLC-QToF- and APGC-QToF-MS reveals metabolic pathways reprogramming in response to tidal cycles in the sub-littoral species Mimachlamys varia exposed to aerial emergence
BT 
AF Ory, Pascaline
   Bonnet, Antoine
   Mondeguer, Florence
   Breitwieser, Marine
   Dubillot, Emmanuel
   Graber, Marianne
AS 1:1;2:1;3:2;4:1;5:1;6:1;
FF 1:;2:;3:PDG-ODE-DYNECO-PHYC;4:;5:;6:;
C1 Littoral Environnement et Sociétés (LIENSs), UMR 7266, CNRS-Université de La Rochelle, 2 rue Olympe de Gouges, F-17042 La Rochelle Cedex 01, France
   Ifremer, Laboratoire Phycotoxines, Rue de l'Ile d'Yeu, 44311 Nantes Cedex, France
C2 UNIV LA ROCHELLE, FRANCE
   IFREMER, FRANCE
SI NANTES
SE PDG-ODE-DYNECO-PHYC
IN WOS Ifremer UPR
   copubli-france
   copubli-univ-france
IF 3.011
TC 6
UR https://archimer.ifremer.fr/doc/00466/57741/59936.pdf
LA English
DT Article
DE ;Anaerobic;APGC-QToF mass spectrometry;Hypoxia;Intertidal;LC-QToF mass spectrometry;Metabolomics;Mimachlamys varia;PLS-DA
AB Mimachlamys varia is a sub-littoral bivalve encountered from Norway to the Mediterranean Sea, which lives mostly byssally attached to rocks. During the low tide period, M. varia individuals, located highest on the shore, may experience short time of aerial exposure and face a low availability of oxygen. Here we report a comparative metabolomic profiling of gill samples of M. varia obtained by both LC-QToF and APGC-QToF mass spectrometry, to analyze metabolic changes occurring during emersion in comparison with immersion. Scallops were grown in aquaria with a simulated intertidal environment mimicking short-duration air exposure that they might experience during extreme tides: alternating 2 h emersion and 10 h immersion. Our results show a switch from aerobic to anaerobic metabolism after only 2 h of emersion, with the resort to different pathways: glucose-lactate, glucose-succinate and aspartate-succinate pathways. Furthermore, carnitine-conjugated metabolites were found to accumulate during emersion, as well as urate. The level of tyrosine on the contrary was found to decrease. These findings indicate a complex metabolic reprogramming that occurs after a two hour emersion period and upon re-immersion. Furthermore, M. varia is used as sentinel species in pollution biomonitoring, through the assay of biomarkers to evaluate the effects of pollutants. Here we show that emersion induces a significant decrease of superoxide dismutase activity, an enzyme developed by bivalves to face oxidative stress and used as biomarker. These findings have to be taken into account to normalize sampling during campaigns of environmental monitoring, by taking in situ, as far as possible only immersed individuals. 
PY 2019
PD MAR
SO Comparative Biochemistry And Physiology D-genomics & Proteomics
SN 1744-117X
PU Elsevier BV
VL 29
UT 000460492500008
BP 74
EP 85
DI 10.1016/j.cbd.2018.11.002
ID 57741
ER

EF