FN Archimer Export Format
PT J
TI Sulphur-oxidizing extracellular bacteria in the gills of Mytilidae associated with wood falls
BT 
AF DUPERRON, Sebastien
   LAURENT, Melina CZ
   GAILL, Francoise
   GROS, Olivier
AS 1:1;2:2;3:1;4:2;
FF 1:;2:;3:;4:;
C1 Univ Paris 06, CNRS, UMR 7138 UPMC IRD MNHN, Paris, France.
   Univ Antilles Guyane, UMR 7138 Syst Adaptat Evolut, Guadeloupe, France.
C2 UNIV PARIS 06, FRANCE
   UNIV ANTILLES GUYANE, FRANCE
IF 3.335
TC 56
UR https://archimer.ifremer.fr/doc/00466/57808/60093.pdf
LA English
DT Article
CR TROPICAL DEEP-SEA BENTHOS
DE ;sulphur-oxidizing bacteria;Bathymodiolus;Idas;Adipicola;sunken woods;Bohol sea
AB Six morphotypes of small mussels (Bivalvia: Mytilidae) were found attached to naturally sunken wood collected in the Bohol Sea (Philippines). These specimens are related to the large Bathymodiolus mussels that are found worldwide at cold seeps and hydrothermal vents. In these habitats, the mytilids harbour sulphur- and methane-oxidizing endosymbionts in their gills and depend on the energy and carbon provided by the symbionts. In this study, bacteria associated with the gills of wood-associated mussels are characterized using molecular and microscopic techniques. The existence of bacteria in the lateral zone of gill filaments in all specimens is demonstrated. Comparative analyses of 16S rRNA gene and adenosine 5'-phosphosulphate (APS) reductase gene sequences indicate that the bacteria are closely related to sulphur-oxidizing endosymbionts of Bathymodiolus. FISHs using specific probes confirm that sulphur oxidizers are by far the most abundant, if not the only bacteria present. Electron micrographs displayed mostly extracellular bacteria located between microvilli at the apical surface of host gill epithelial cells all along the lateral zone of each gill filament. In some specimens, occasional occurrence of intracellular bacteria with similar morphology was noted. This study provides the first molecular evidence for the presence of possible thiotrophic symbiosis in sunken wood ecosystems. With their epibiotic bacteria, wood-associated mussels display a less integrated type of interaction than described in their seep, vent and whale fall relatives. 
PY 2008
PD MAR
SO Fems Microbiology Ecology
SN 0168-6496
PU Blackwell Publishing
VL 63
IS 3
UT 000252966300008
BP 338
EP 349
DI 10.1111/j.1574-6941.2008.00438.x
ID 57808
ER

EF