Litopenaeus vannamei stylicins are constitutively produced by hemocytes and intestinal cells and are differentially modulated upon infections

Type Article
Date 2019-03
Language English
Author(s) Farias Natanael Dantas1, Falchetti Marcelo1, Matos Gabriel Machado1, Schmitt Paulina2, Barreto Cairé1, Argenta Nicolas1, Rolland Jean-Luc3, Bachère Evelyne3, Perazzolo Luciane Maria1, Rosa Rafael Diego1
Affiliation(s) 1 : Laboratory of Immunology Applied to Aquaculture, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, 88040-900, Florianópolis, SC, Brazil
2 : Laboratorio de Genética e Inmunología Molecular, Instituto de Biología, Facultad de Ciencias, Pontificia Universidad Católica de Valparaíso, 2373223, Valparaíso, Chile
3 : Univ Perpignan Via Domitia, IFREMER, Univ Montpellier,CNRS, Interact Hotes Pathogenes Environm, F-34090 Montpellier 5, France
Source Fish & Shellfish Immunology (1050-4648) (Elsevier BV), 2019-03 , Vol. 86 , P. 82-92
DOI 10.1016/j.fsi.2018.11.021
WOS© Times Cited 5
Keyword(s) Crustacean, Penaeid shrimp, Invertebrate immunity, Antimicrobial peptide, Host defense peptide, Molecular diversity
Abstract

Stylicins are anionic antimicrobial host defense peptides (AAMPs) composed of a proline-rich N-terminal region and a C-terminal portion containing 13 conserved cysteine residues. Here, we have increased our knowledge about these unexplored crustacean AAMPs by the characterization of novel stylicin members in the most cultivated penaeid shrimp, Litopenaeus vannamei. We showed that the L. vannamei stylicin family is composed of two members (Lvan-Stylicin1 and Lvan-Stylicin2) encoded by different loci which vary in gene copy number. Unlike the other three gene-encoded antimicrobial peptide families from penaeid shrimp, the expression of Lvan-Stylicins is not restricted to hemocytes. Indeed, they are also produced by the columnar epithelial cells lining the midgut and its anterior caecum. Interestingly, Lvan-Stylicins are simultaneously transcribed at different transcriptional levels in a single shrimp and are differentially modulated in hemocytes after infections. While the expression of both genes showed to be responsive to damage-associated molecular patterns, only Lvan-Stylicin2 was induced after a Vibrio infection. Besides, Lvan-Stylicins also showed a distinct pattern of gene expression in the three portions of the midgut (anterior, middle and posterior) and during shrimp development. We provide here the first evidence of the diversity of the stylicin antimicrobial peptide family in terms of sequence and gene expression distribution and regulation

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Farias Natanael Dantas, Falchetti Marcelo, Matos Gabriel Machado, Schmitt Paulina, Barreto Cairé, Argenta Nicolas, Rolland Jean-Luc, Bachère Evelyne, Perazzolo Luciane Maria, Rosa Rafael Diego (2019). Litopenaeus vannamei stylicins are constitutively produced by hemocytes and intestinal cells and are differentially modulated upon infections. Fish & Shellfish Immunology, 86, 82-92. Publisher's official version : https://doi.org/10.1016/j.fsi.2018.11.021 , Open Access version : https://archimer.ifremer.fr/doc/00466/57816/