FN Archimer Export Format PT J TI Litopenaeus vannamei stylicins are constitutively produced by hemocytes and intestinal cells and are differentially modulated upon infections BT AF Farias, Natanael Dantas Falchetti, Marcelo Matos, Gabriel Machado Schmitt, Paulina Barreto, Cairé Argenta, Nicolas Rolland, Jean-Luc Bachère, Evelyne Perazzolo, Luciane Maria Rosa, Rafael Diego AS 1:1;2:1;3:1;4:2;5:1;6:1;7:3;8:3;9:1;10:1; FF 1:;2:;3:;4:;5:;6:;7:PDG-RBE-IHPE;8:PDG-RBE-IHPE;9:;10:; C1 Laboratory of Immunology Applied to Aquaculture, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, 88040-900, Florianópolis, SC, Brazil Laboratorio de Genética e Inmunología Molecular, Instituto de Biología, Facultad de Ciencias, Pontificia Universidad Católica de Valparaíso, 2373223, Valparaíso, Chile Univ Perpignan Via Domitia, IFREMER, Univ Montpellier,CNRS, Interact Hotes Pathogenes Environm, F-34090 Montpellier 5, France C2 UNIV FED SANTA CATARINA, BRAZIL UNIV PONTIFICIA CATOLICA VALPARAISO, CHILE IFREMER, FRANCE SI MONTPELLIER SE PDG-RBE-IHPE UM IHPE IN WOS Ifremer UMR copubli-int-hors-europe copubli-sud IF 1.618 TC 11 UR https://archimer.ifremer.fr/doc/00466/57816/60102.pdf LA English DT Article DE ;Crustacean;Penaeid shrimp;Invertebrate immunity;Antimicrobial peptide;Host defense peptide;Molecular diversity AB Stylicins are anionic antimicrobial host defense peptides (AAMPs) composed of a proline-rich N-terminal region and a C-terminal portion containing 13 conserved cysteine residues. Here, we have increased our knowledge about these unexplored crustacean AAMPs by the characterization of novel stylicin members in the most cultivated penaeid shrimp, Litopenaeus vannamei. We showed that the L. vannamei stylicin family is composed of two members (Lvan-Stylicin1 and Lvan-Stylicin2) encoded by different loci which vary in gene copy number. Unlike the other three gene-encoded antimicrobial peptide families from penaeid shrimp, the expression of Lvan-Stylicins is not restricted to hemocytes. Indeed, they are also produced by the columnar epithelial cells lining the midgut and its anterior caecum. Interestingly, Lvan-Stylicins are simultaneously transcribed at different transcriptional levels in a single shrimp and are differentially modulated in hemocytes after infections. While the expression of both genes showed to be responsive to damage-associated molecular patterns, only Lvan-Stylicin2 was induced after a Vibrio infection. Besides, Lvan-Stylicins also showed a distinct pattern of gene expression in the three portions of the midgut (anterior, middle and posterior) and during shrimp development. We provide here the first evidence of the diversity of the stylicin antimicrobial peptide family in terms of sequence and gene expression distribution and regulation PY 2019 PD MAR SO Fish & Shellfish Immunology SN 1050-4648 PU Elsevier BV VL 86 UT 000459528400010 BP 82 EP 92 DI 10.1016/j.fsi.2018.11.021 ID 57816 ER EF