FN Archimer Export Format PT J TI CO I Barcoding Reveals New Clades and Radiation Patterns of Indo-Pacific Sponges of the Family Irciniidae (Demospongiae: Dictyoceratida) BT AF POEPPE, Judith SUTCLIFFE, Patricia HOOPER, John N. A. WOERHEIDE, Gert ERPENBECK, Dirk AS 1:1;2:2;3:2;4:3,4;5:3,4; FF 1:;2:;3:;4:;5:; C1 Humboldt Univ, Museum Nat Kunde, Dept Malacozool, Leibniz Inst Res Evolut & Biodivers, Berlin, Germany. Queensland Museum, Biodivers Program, Brisbane, Australia. Univ Munich, Dept Earth & Environm Sci Palaeontol & Geobiol, Munich, Germany. Univ Munich, GeoBioCtr, Munich, Germany. C2 UNIV HUMBOLDT, GERMANY QUEENSLAND MUSEUM, AUSTRALIA UNIV MUNICH, GERMANY UNIV MUNICH, GERMANY IN DOAJ IF 4.411 TC 36 UR https://archimer.ifremer.fr/doc/00467/57838/60121.pdf https://archimer.ifremer.fr/doc/00467/57838/60122.pdf https://archimer.ifremer.fr/doc/00467/57838/60123.pdf https://archimer.ifremer.fr/doc/00467/57838/60124.pdf LA English DT Article CR TROPICAL DEEP-SEA BENTHOS AB Background: DNA barcoding is a promising tool to facilitate a rapid and unambiguous identification of sponge species. Demosponges of the order Dictyoceratida are particularly challenging to identify, but are of ecological as well as biochemical importance. Methodology/Principal Findings: Here we apply DNA barcoding with the standard CO1-barcoding marker on selected Indo-Pacific specimens of two genera, Ircinia and Psammocinia of the family Irciniidae. We show that the CO1 marker identifies several species new to science, reveals separate radiation patterns of deep-sea Ircinia sponges and indicates dispersal patterns of Psammocinia species. However, some species cannot be unambiguously barcoded by solely this marker due to low evolutionary rates. Conclusions/Significance: We support previous suggestions for a combination of the standard CO1 fragment with an additional fragment for sponge DNA barcoding. PY 2010 PD APR SO Plos One SN 1932-6203 PU Public Library Science VL 5 IS 3 UT 000276418200026 DI 10.1371/journal.pone.0009950 ID 57838 ER EF