FN Archimer Export Format
PT J
TI Effects of extraction method and storage of dry tissue on marine lipids and fatty acids
BT 
AF SARDENNE, Fany
   BODIN, Nathalie
   METRAL, Luisa
   CROTTIER, Anais
   LE GRAND, Fabienne
   BIDEAU, Antoine
   BRISSET, Blandine
   BOURJEA, Jerome
   SARAUX, Claire
   BONHOMMEAU, Sylvain
   KERZERHO, Vincent
   BERNARD, Serge
   ROUYER, Tristan
AS 1:1;2:2;3:1;4:1;5:3,6;6:3;7:1;8:1;9:1;10:4;11:5;12:5;13:1;
FF 1:PDG-RBE-MARBEC-LHM;2:;3:PDG-RBE-MARBEC-LHM;4:PDG-ODE-LITTORAL-LERLR;5:;6:;7:PDG-RBE-MARBEC-LHM;8:PDG-RBE-MARBEC-LHM;9:PDG-RBE-MARBEC-LHM;10:PDG-RBE-DOI;11:;12:;13:PDG-RBE-MARBEC-LHM;
C1 MARBEC Univ Montpellier, IRD, Ifremer, CNRS, Sete, France.
   Seychelles Fishing Author, Victoria, Seychelles.
   Ifremer, UBO, LEMAR CNRS, IRD, Brest, France.
   Ifremer DOI, Le Port, La Reunion, France.
   LIRMM Univ Montpellier, CNRS, Montpellier, France.
C2 IFREMER, FRANCE
   SEYCHELLES FISHING AUTHOR, SEYCHELLES
   UBO, FRANCE
   IFREMER, FRANCE
   UNIV MONTPELLIER, FRANCE
   CNRS, FRANCE
SI SETE
   LA REUNION
SE PDG-RBE-MARBEC-LHM
   PDG-ODE-LITTORAL-LERLR
   PDG-RBE-DOI
UM LEMAR
   MARBEC
IN WOS Ifremer UPR
   WOS Ifremer UMR
   WOS Cotutelle UMR
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
IF 5.977
TC 21
UR https://archimer.ifremer.fr/doc/00467/57898/60189.pdf
LA English
DT Article
DE ;Degradation;Freeze-drying;Lyophilization;Lipid class;Marine animal;Protocol
AB Various protocols are currently used to study marine lipids, but there is a growing interest in working on dry samples that are easier to transport. However, reference protocols are still lacking for dry samples. In order to make recommendations on this use, lipid classes and fatty acids (FA) obtained from six analytical protocols using two different tissue states (dry vs wet) and three extraction methods (automat vs manual potter vs leaving the solvent to work on tissue) were compared. Three dry storage modes of tissue (freezer vs gas nitrogen vs dry room) during one and three months were also compared. These comparisons were made on seven marine species with different lipid profiles, including fishes, crustaceans and mollusks. Lipid classes and FA obtained from wet and dry tissues were similar, but they were affected by the extraction methods. Regardless of tissue state, “Leave to work” methods obtained the highest lipid quantities, followed by manual potter and automat methods (ca. 90% and 80% of “Leave to work” methods, respectively). Linear relationships allowed correction for lipid classes and FA concentrations obtained from different protocols. The repeatability of all protocols still needs to be improved, especially for fish species. Increasing the replicate number for each sample might be an indirect way to improve lipid quantification. Our results show that storing dry tissues in the freezer for more than one month was associated with a decrease in lipids, which is also observed for other storage methods. For qualitative studies of FA (expressed in %), a three-month storage of dry tissue in freezer did not affect the relative composition of species/tissues with a lipid content below 20% of dry weight. 
PY 2019
PD MAR
SO Analytica Chimica Acta
SN 0003-2670
PU Elsevier Science Bv
VL 1051
UT 000455934900008
BP 82
EP 93
DI 10.1016/j.aca.2018.11.012
ID 57898
ER

EF