TY - JOUR T1 - Relation between Biofilm and Virulence in Vibrio tapetis: A Transcriptomic Study A1 - Rodrigues,Sophie A1 - Paillard,Christine A1 - Van Dillen,Sabine A1 - Tahrioui,Ali A1 - Berjeaud,Jean-Marc A1 - Dufour,Alain A1 - Bazire,Alexis AD - IUEM Univ Bretagne Sud, LBCM, EA 3884, F-56100 Lorient, France. AD - Univ Brest, UBO, Lab Sci Environm Marin LEMAR, UMR6539,CNRS,IRD,Ifremer,Inst Univ Europeen Mer, F-29280 Plouzane, France. AD - Danisco France SAS, DuPont Nutr & Hlth, BP10, F-86220 Dange St Romain, France. AD - Univ Rouen Normandy, LMSM EA 4312, F-27000 Evreux, France. AD - Univ Poitiers, Lab Ecol & Biol Interact EBI, UMR 7267, F-86000 Poitiers, France. UR - https://archimer.ifremer.fr/doc/00470/58140/ DO - 10.3390/pathogens7040092 KW - biofilm KW - virulence KW - Vibrio tapetis KW - transcriptome KW - quorum sensing KW - type VI secretion system KW - brown ring disease N2 - Marine pathogenic bacteria are able to form biofilms on many surfaces, such as mollusc shells, and they can wait for the appropriate opportunity to induce their virulence. Vibrio tapetis can develop such biofilms on the inner surface of shells of the Ruditapes philippinarum clam, leading to the formation of a brown conchiolin deposit in the form of a ring, hence the name of the disease: Brown Ring Disease. The virulence of V. tapetis is presumed to be related to its capacity to form biofilms, but the link has never been clearly established at the physiological or genetic level. In the present study, we used RNA-seq analysis to identify biofilm- and virulence-related genes displaying altered expression in biofilms compared to the planktonic condition. A flow cell system was employed to grow biofilms to obtain both structural and transcriptomic views of the biofilms. We found that 3615 genes were differentially expressed, confirming that biofilm and planktonic lifestyles are very different. As expected, the differentially expressed genes included those involved in biofilm formation, such as motility- and polysaccharide synthesis-related genes. The data show that quorum sensing is probably mediated by the AI-2/LuxO system in V. tapetis biofilms. The expression of genes encoding the Type VI Secretion System and associated exported proteins are strongly induced, suggesting that V. tapetis activates this virulence factor when living in biofilm Y1 - 2018/12 PB - Mdpi JF - Pathogens SN - 2076-0817 VL - 7 IS - 4 ID - 58140 ER -