FN Archimer Export Format
PT J
TI Whole transcriptome sequencing and biomineralization gene architecture associated with cultured pearl quality traits in the pearl oyster, Pinctada margaritifera
BT 
AF LE LUYER, Jeremy
   AUFFRET, Pauline
   QUILLIEN, Virgile
   LECLERC, Nicolas
   REISSER, Celine
   VIDAL-DUPIOL, Jeremie
   KY, Chin-Long
AS 1:1;2:1;3:1;4:1;5:1;6:1,2;7:1;
FF 1:PDG-RBE-RMPF;2:PDG-RBE-RMPF;3:PDG-RBE-RMPF;4:PDG-RBE-RMPF;5:PDG-RBE-RMPF;6:PDG-RBE-IHPE;7:PDG-RBE-RMPF;
C1 Ctr Ifremer Pacifique, Labex Corail, EIO, IFREMER,UMR 241, BP 49, F-98719 Tahiti, French Polynesi, France.
   Univ Montpellier, IFREMER, UMR Interact Hotes Pathogenes Environm 5244, Pl Eugene Bataillon CC 80, F-34095 Montpellier, France.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
SI TAHITI
   MONTPELLIER
SE PDG-RBE-RMPF
   PDG-RBE-IHPE
UM IHPE
   EIO
IN WOS Ifremer UMR
   DOAJ
IF 3.594
TC 17
UR https://archimer.ifremer.fr/doc/00475/58695/61207.pdf
   https://archimer.ifremer.fr/doc/00475/58695/62104.pdf
LA English
DT Article
DE ;Pinctada margaritifera;Biomineralization;Pearl quality;Pearl sac;RNA-seq
AB Background Cultured pearls are unique gems produced by living organisms, mainly molluscs of the  Pinctada genus, through the biomineralization properties of pearl sac tissue.  Improvement of P. margaritifera pearl quality is one of the biggest challenges that  Polynesian research has faced to date. To achieve this goal, a better understanding of the  complex mechanisms related to nacre and pearl formation is essential and can now be  approached through the use of massive parallel sequencing technologies. The aim of this  study was to use RNA-seq to compare whole transcriptome expression of pearl sacs that  had producing pearls with high and low quality. For this purpose, a comprehensive  reference transcriptome of P. margaritifera was built based on multi-tissue sampling  (mantle, gonad, whole animal), including different living stages (juvenile, adults) and  phenotypes (colour morphotypes, sex). Results Strikingly, few genes were found to be up-regulated for high quality pearls (n = 16)  compared to the up-regulated genes in low quality pearls (n = 246). Biomineralization  genes up-regulated in low quality pearls were specific to prismatic and prism-nacre  layers. Alternative splicing was further identified in several key biomineralization genes  based on a recent P. margaritifera draft genome. Conclusion This study lifts the veil on the multi-level regulation of biomineralization genes 38 associated with pearl quality determination. 
PY 2019
PD FEB
SO Bmc Genomics
SN 1471-2164
PU Bmc
VL 20
UT 000458370700006
DI 10.1186/s12864-019-5443-5
ID 58695
ER

EF