FN Archimer Export Format
PT J
TI Ontogeny of the digestive enzyme activity of the Amazonian pimelodid catfish Pseudoplatystoma punctifer (Castelnau, 1855)
BT 
AF Castro-Ruiz, Diana
   Mozanzadeh, Mansour Torfi
   Fernández-Méndez, Christian
   Andree, Karl B.
   García-Dávila, Carmen
   Cahu, Chantal
   Gisbert, Enric
   Darias, Maria J.
AS 1:1;2:2;3:1;4:3;5:1;6:4;7:3;8:5;
FF 1:;2:;3:;4:;5:;6:PDG-RBE-PFOM;7:;8:;
C1 Instituto de Investigaciones de la Amazonía Peruana (IIAP), Programa de Investigación para el Uso y Conservación del Agua y sus Recursos, Iquitos, Peru
   Department of Fisheries, South Iran Aquaculture Research Center, Marine Fish Aquaculture Research Station, Bandar Imam, Khomeyni, Khozestan, Iran
   Institut de Recerca i Tecnologia Agroalimentaries (IRTA), Centre de Sant Carles de la Ràpita (IRTA-SCR), Unitat de Cultius Aqüícoles, Sant Carles de la Ràpita, Spain
   IFREMER, UMR Laboratoire des Sciences de l'Environnement Marin, Plouzané, France
   MARBEC, IRD, Univ. Montpellier, CNRS, Ifremer, Montpellier, France
C2 IIAP, PERU
   IFRO, IRAN
   IRTA, SPAIN
   IFREMER, FRANCE
   IRD, FRANCE
SI BREST
SE PDG-RBE-PFOM
UM LEMAR
   MARBEC
IN WOS Ifremer UMR
   WOS Cotutelle UMR
   copubli-france
   copubli-p187
   copubli-europe
   copubli-int-hors-europe
   copubli-sud
IF 3.224
TC 17
UR https://archimer.ifremer.fr/doc/00479/59082/61723.pdf
LA English
DT Article
DE ;Pseudoplatystoma punctifer;Ontogeny;Enzymatic activity;Digestive system;Brush border;Pancreatic enzymes;Gastric enzymes
AB The aim of the study was to evaluate the functional ontogeny of the digestive system of Pseudoplatystoma punctifer through the analysis of the activity of the main intestinal (alkaline phosphatase, aminopeptidase N, maltase and leucine-alanine peptidase), pancreatic (trypsin, chymotrypsin, total alkaline proteases, bile-salt activated lipase and amylase) and gastric (pepsin) enzymes. Larvae were raised in triplicate in a recirculation system from 4 to 27 days post fertilization (dpf) at an initial density of 90 larvae L−1, 27.8 ± 0.7 °C and 0 L: 24D photoperiod. Larvae were fed from 4 to 17 dpf with Artemia nauplii and weaned onto an experimentally formulated feed (crude protein content ~ 45%; crude fat content ~ 10%; crude carbohydrate ~ 8%) within 3 days, then continued with the same diet until the end of the trial. P. punctifer showed an exponential growth pattern with two different growth rates: a slower one from hatching to 12 dpf followed by a faster one from 12 to 27 dpf. The specific and total activities of the pancreatic and intestinal enzymes were detected from hatching. The digestive system was functional at 12 dpf, indicating the transition from the larval to the juvenile stage (alkaline to acid digestion). Therefore individuals could be weaned from that day onwards. The variations observed in the enzymatic activity from 17 dpf reflected the adaptation of the enzymatic machinery to the new diet supplied. P. punctifer larvae showed a fast digestive system development with an enzymatic profile typical of a tropical and carnivorous species. 
PY 2019
PD APR
SO Aquaculture
SN 0044-8486
PU Elsevier BV
VL 504
UT 000459962400026
BP 210
EP 218
DI 10.1016/j.aquaculture.2019.01.059
ID 59082
ER

EF