FN Archimer Export Format
PT J
TI Inter and intra-specific transcriptional and phenotypic responses of   Pseudo-nitzschia   under different nutrient conditions
BT 
AF LEMA, Anais Kimberley
   Metegnier, Gabriel
   Quéré, Julien
   Latimier, Marie
   Youenou, Agnes
   Lambert, Christophe
   Fauchot, Juliette
   Le Gac, Mickael
AS 1:1;2:1,2;3:1;4:1;5:1;6:3;7:4;8:1;
FF 1:PDG-ODE-DYNECO-PELAGOS;2:PDG-ODE-DYNECO-PELAGOS;3:PDG-ODE-DYNECO-PELAGOS;4:PDG-ODE-DYNECO-PELAGOS;5:PDG-ODE-DYNECO-PELAGOS;6:;7:;8:PDG-ODE-DYNECO-PELAGOS;
C1 IFREMER, Dyneco Pelagos, Plouzané, France
   CNRS, Sorbonne Université, Pontificia Universidad Catolica de Chile, Universidad Austral de Chile, UMI 3614, Evolutionary Biology and Ecology of Algae, Station Biologique de Roscoff, Place Georges Teissier, CS90074 29688, Roscoff Cedex, France
   Laboratoire des Sciences de l’Environnement Marin (LEMAR), UMR 6539 CNRS UBO IRD IFREMER; Institut Universitaire Européen de la Mer, Technopôle Brest-Iroise, Rue Dumont d’Urville, 29280 Plouzané, France
   Normandie Univ, UNICAEN, CNRS, BOREA, Caen, France
C2 IFREMER, FRANCE
   CNRS, FRANCE
   CNRS, FRANCE
   UNIV NORMANDIE, FRANCE
SI BREST
SE PDG-ODE-DYNECO-PELAGOS
UM LEMAR
IN WOS Ifremer UPR
   WOS Cotutelle UMR
   DOAJ
   copubli-france
   copubli-univ-france
IF 3.462
TC 5
UR https://archimer.ifremer.fr/doc/00482/59392/62157.pdf
   https://archimer.ifremer.fr/doc/00482/59392/62158.pdf
LA English
DT Article
DE ;phenotype;transcriptomics;Pseudo-nitzschia;divergence;nutrients
AB Untangling the functional basis of divergence between closely related species is a step towards understanding species dynamics within communities at both the evolutionary and ecological scales. We investigated cellular (i.e: growth, domoic acid production, nutrient consumption) and molecular (transcriptomic analyses) responses to varying nutrient concentrations across several strains belonging to three species of the toxic diatom genus Pseudo-nitzschia. Three main results were obtained. First, strains from the same species displayed similar transcriptomic, but not necessarily cellular, responses to the experimental conditions. It showed the importance of considering intra-specific diversity to investigate functional divergence between species. Second, a major exception to the first finding was a strain recently isolated from the natural environment and displaying contrasting gene expression patterns related to cell motility and domoic acid production. This result illustrated the profound modifications that may occur when transferring a cell from the natural to the in vitro environment, and asks for future studies to better understand the influence of culture duration and life cycle on expression patterns. Third, transcriptomic responses were more similar between the two species displaying similar ecology in situ, irrespective of the genetic distance. This was especially true for molecular responses related to TCA cycle, photosynthesis, and nitrogen metabolism. However, transcripts related to phosphate uptake were variable between species. It highlighted the importance of considering both overall genetic distance and ecological divergence to explain functional divergence between species. 
PY 2019
PD MAR
SO Genome Biology And Evolution
SN 1759-6653
PU Oxford University Press (OUP)
VL 11
IS 3
UT 000476569300010
BP 731
EP 747
DI 10.1093/gbe/evz030
ID 59392
ER

EF